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Instantaneous Iodine-Assisted DNAzyme Cleavage of Phosphorothioate RNA

Description: We show that Ce13d DNAzymes can use non-electrostatic interactions to assist RNA cleavage by making simple Phosphorothioate (PS) modifications at the cleavage junction. In this case, Iodine can replace the role of a metal cofactor, acting directly on the scissile phosphate group. This finding has broadened the scope of DNAzyme catalysis. This DNAzyme was further engineered for fluorescence-based I2 detection with a detection limit of 4.7 nM. In addition, Oxidation of I− with Fe3+ produced I2 in situ, allowing detection of Fe3+ down to 78 nM.
Authors: Huang, Po-Jung Jimmy; Water Institute; University of Waterloo; ORCID iD 0000-0003-3436-9968
Moon, Woohyun Jennifer; University of Waterloo
Liu, Juewen; Water Institute; University of Waterloo; ORCID iD 0000-0001-5918-9336
Keywords: DNAzyme
DNA
Biosensor
Fluorescence
Iodine
Field of Research: 
Biological sciences
>
Biochemistry
>
Analytical biochemistry
Publication Date: 2021-03-08
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Natural Sciences and Engineering Research Council of Canada (NSERC)
Canada First Research Excellence Fund (CFREF); Global Water Futures Program (GWF)
URI: https://doi.org/10.20383/102.0326
Related Identifiers: 
This dataset is a supplement to
https://doi.org/10.1021/acs.biochem.8b00900
Appears in Collections:Water Institute

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Huang, P., Moon, W., Liu, J. (2021). Instantaneous Iodine-Assisted DNAzyme Cleavage of Phosphorothioate RNA. Federated Research Data Repository. https://doi.org/10.20383/102.0326

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