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Instantaneous Iodine-Assisted DNAzyme Cleavage of Phosphorothioate RNA
| Description: | We show that Ce13d DNAzymes can use non-electrostatic interactions to assist RNA cleavage by making simple Phosphorothioate (PS) modifications at the cleavage junction. In this case, Iodine can replace the role of a metal cofactor, acting directly on the scissile phosphate group. This finding has broadened the scope of DNAzyme catalysis. This DNAzyme was further engineered for fluorescence-based I2 detection with a detection limit of 4.7 nM. In addition, Oxidation of I− with Fe3+ produced I2 in situ, allowing detection of Fe3+ down to 78 nM. |
| Authors: | Huang, Po-Jung Jimmy; Water Institute; University of Waterloo;  0000-0003-3436-9968Moon, Woohyun Jennifer; University of Waterloo Liu, Juewen; Water Institute; University of Waterloo; 0000-0001-5918-9336 |
| Keywords: | DNAzyme DNA Biosensor Fluorescence Iodine |
| Field of Research: | Biological sciences > Biochemistry > Analytical biochemistry
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| Publication Date: | 2021-03-08 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council of Canada (NSERC) Canada First Research Excellence Fund (CFREF); Global Water Futures Program (GWF) |
| URI: | https://doi.org/10.20383/102.0326 |
| Related Identifiers: |
This dataset is a supplement to
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| Appears in Collections: | Water Institute |
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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Huang, P., Moon, W., Liu, J. (2021). Instantaneous Iodine-Assisted DNAzyme Cleavage of Phosphorothioate RNA. Federated Research Data Repository. https://doi.org/10.20383/102.0326
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