Field
Value
Language
dc.contributor.author
Huang, Po-Jung Jimmy
datacite.creator.affiliationIdentifier
-;https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
Water Institute; University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-3436-9968
en_US
dc.contributor.author
Moon, Woohyun Jennifer
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Liu, Juewen
datacite.creator.affiliationIdentifier
-;https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
Water Institute; University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.date.accessioned
2021-03-08T21:10:45Z
dc.date.available
2021-03-08T21:10:45Z
dc.date.issued
2021-03-08
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/080af169-c2a9-4b1a-934e-d63e16db76d5
dc.identifier.uri
https://doi.org/10.20383/102.0326
dc.description
We show that Ce13d DNAzymes can use non-electrostatic interactions to assist RNA cleavage by making simple Phosphorothioate (PS) modifications at the cleavage junction. In this case, Iodine can replace the role of a metal cofactor, acting directly on the scissile phosphate group. This finding has broadened the scope of DNAzyme catalysis. This DNAzyme was further engineered for fluorescence-based I2 detection with a detection limit of 4.7 nM. In addition, Oxidation of I− with Fe3+ produced I2 in situ, allowing detection of Fe3+ down to 78 nM.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution 4.0 International (CC BY 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
en_US
dc.subject
DNAzyme
en_US
dc.subject
DNA
en_US
dc.subject
Biosensor
en_US
dc.subject
Fluorescence
en_US
dc.subject
Iodine
en_US
dc.title
Instantaneous Iodine-Assisted DNAzyme Cleavage of Phosphorothioate RNA
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_321/
frdr.preservation.status
AIP generation and transfer successful
frdr.preservation.datetime
2022-02-02
datacite.publicationyear
2021
datacite.date.Collected
2018-02-01/2018-08-01
datacite.resourcetype
Dataset
en_US
datacite.relatedidentifier.IsSupplementTo
https://doi.org/10.1021/acs.biochem.8b00900
datacite.fundingReference.funderIdentifier
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada (NSERC)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
datacite.fundingReference.funderName
Canada First Research Excellence Fund (CFREF)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
Global Water Futures Program (GWF)
en_US
datacite.fundingReference.funderName
datacite.fundingReference.awardNumber
datacite.fundingReference.awardTitle
datacite.fundingReference.funderName
datacite.fundingReference.awardNumber
datacite.fundingReference.awardTitle
frdr.crdc.code
RDF1060101
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
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