Oxidation of Fe(II) by Flavins under Anoxic Conditions

Zhang, Peng

-;https://ror.org/01aff2v68;-;https://ror.org/04gcegc37

Water Institute; University of Waterloo; State Key Laboratory of Biogeology and Environmental Geology; China University of Geosciences

https://orcid.org/0000-0003-2664-9601

Van Cappellen, Philippe

-;https://ror.org/01aff2v68

Water Institute; University of Waterloo

https://orcid.org/0000-0001-5476-082

Pi, Kunfu

-;https://ror.org/01aff2v68

Water Institute; University of Waterloo

https://orcid.org/0000-0002-0798-083

2021-02-16T17:09:39Z

2021-02-16T17:09:39Z

2021-02-16

https://www.frdr-dfdr.ca/repo/dataset/12f19b1d-c11d-4d18-b9db-58309ed13503

https://doi.org/10.20383/102.0320

This project aims to understand the redox reaction between Fe(II) and flavins. Our study demonstrated that aqueous Fe(II) can be oxidized by the oxidized form of riboflavin and flavin mononucleotide at pH 7–9. Both the rates and extents of oxidation increased with increasing concentration of oxidized flavins and increasing solution pH.

Our data are from experiments, which mainly consist of the chemical variables, were collected in the Ecohydrology lab at the University of Waterloo using the batch reaction system. All the experiments were conducted in 125 mL serum bottles at 22±1 ℃ in an anaerobic glovebox (95% N2 and 5% H2). The bottles contained Teflon-coated magnetic stir bars and were wrapped in aluminum foil to exclude light. Prior to an experiment, a solution containing 20 mM buffer and 10 mM NaCl was purged with N2 (99.998%) for at least 2 h to remove O2, then transferred to the anaerobic glovebox. Aqueous Fe2+ and uncomplexed RBF and FMN concentrations were analyzed by using a Flexstation Multimode Microplate Reader. The total concentrations of RBF and FMN were determined at 450 nm on a UV-vis spectrometer kept in the anaerobic glovebox

Additional details on the project are available in Environmental Science and Technology publication titled "Oxidation of Fe(II) by flavins under anoxic conditions" (doi: 10.1021/acs.est.0c02916)

Federated Research Data Repository / dépôt fédéré de données de recherche

Creative Commons Attribution 4.0 International (CC BY 4.0)

https://creativecommons.org/licenses/by/4.0/

Fe(II)

flavins

oxidation

alkaline

pH

Oxidation of Fe(II) by Flavins under Anoxic Conditions

f163c1b3-9c88-42f6-a7bb-5839ed6c4063

/2/published/publication_315/

Declined

AIP generation and transfer successful

2022-02-02

2021

2017-11-01/2018-02-01

Dataset

https://doi.org/10.1021/acs.est.0c02916

Canada Excellence Research Chairs (CERC)

Canada Excellence Research Chair in Ecohydrology

Natural Sciences and Engineering Research Council of Canada (NSERC)

STPGP-494652-16

National Natural Science Foundation of China

RDF1050208

Earth and related environmental sciences

Geochemistry

Environmental geochemistry

Sciences de la Terre et sciences de l'environnement connexes

Géochimie

Géochimie de l'environnement