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RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures
| Description: | Zooplankton metabarcoding can provide a high-throughput method for characterizing community response to environmental stressors. There are issues, however, with inferring actual response to stressors using DNA metabarcoding as it cannot distinguish alive organisms within bulk samples. Here we used normalized vitality, namely RNA metabarcoding normalized by DNA metabarcoding, to characterize the zooplankton community response to environmental influence. DNA and RNA metabarcoding was also applied in the context of assessing response of the zooplankton community exposed to simulated spills of diluted bitumen (dilbit), with experimental remediation practices of enhanced monitored natural recovery and shoreline cleaner application. Zooplankton samples were collected via pump on days -3 and 11 and 38 days after the simulated dilbit spill. The zooplankton samples were co-extracted for DNA and RNA and were PCR amplified targeting the mitochondrial Cytochrome c Oxidase subunit I gene (CO1) region, with amplicon sequencing following. The dataset includes the demultiplexed sequencing output, the feature table with species-level taxonomic annotation, and the sample metadata. This dataset contains data from enclosures in rock and cobble substrate. Note that a similar study was conducted for wetland habitat enclosures, with different analyses and interpretation being conducted on the data (dataset available at https://doi.org/10.20383/101.0313). |
| Notes: | Item exited embargo and became publicly available on 2021-12-01 |
| Authors: | Ankley, Phillip; University of Saskatchewan;  0000-0002-5883-6148Xie, Yuwei; University of Saskatchewan; 0000-0001-5652-6413Havens, Sonya; International Institute for Sustainable Development Peters, Lisa; University of Manitoba; 0000-0002-4310-3136Timlick, Lauren; International Institute for Sustainable Development Rodriguez-Gil, Jose Luis; International Institute for Sustainable Development; 0000-0001-9325-9131Giesy, John; University of Saskatchewan; 0000-0003-1931-9962Palace, Vince; International Institute for Sustainable Development; 0000-0003-2134-2299 |
| Keywords: | metabarcoding zooplankton ecogenomics Cytochrome c Oxidase subunit 1 diluted bitumen remediation boreal freshwater community RNA |
| Field of Research: | Biological sciences > Ecology (except applications) > Environmental metagenomics
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| Publication Date: | 2021-11-01 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Canada First Research Excellence Fund (CFREF); Global Water Futures Program (GWF); 419205 Global Water Futures (GWF) Global Water Futures (GWF); Next generation solutions to ensure healthy water resources for future generations |
| URI: | https://doi.org/10.20383/102.0332 |
| Related Identifiers: |
This dataset is cited by
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| Geographic Coverage: | Place Name ELA Lake 260 Province / Province / Territory Ontario Territory Country Canada |
| Geographic Point: | Latitude 49.6964 Longitude -93.76752 |
| Appears in Collections: | Global Water Futures |
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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Ankley, P., Xie, Y., Havens, S., Peters, L., Timlick, L., Rodriguez-Gil, J., Giesy, J., Palace, V. (2021). RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures. Federated Research Data Repository. https://doi.org/10.20383/102.0332
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