Field
Value
Language
dc.contributor.author
Haskell, Blake R.
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0009-0002-6620-2070
en_US
dc.contributor.author
Dhiyebi, Hadi A.
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0002-8101-8960
en_US
dc.contributor.author
Servos, Mark
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0002-3783-627X
en_US
dc.coverage.temporal
2021-08-18/2022-04-29
dc.date.accessioned
2023-12-13T21:53:12Z
dc.date.available
2023-12-13T21:53:12Z
dc.date.issued
2023-12-13
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/1d667f1a-28cf-42fd-818b-9bec2d662e45
dc.identifier.uri
https://doi.org/10.20383/103.0856
dc.description
Wastewater-based surveillance (WBS) has been increasingly applied at sites upstream of the wastewater treatment plant (WWTP) to monitoring SARS-CoV-2 RNA at the building-scale. The dataset described is the result of an eight-month long passive sampling campaign on the University of Waterloo (Waterloo, ON) campus. The goal was to determine if passive sampling could support institution-level decision-making. In brief, the method involved the 24-hour deployment of cotton gauze passive samplers enclosed in a 3D printed housing vessel. Upon collection, passive sampling materials were washed and the resulting solids were concentrated. RNA extraction from solids was performed using a kit-based extraction method. Two regions of the SARS-CoV-2 nucleocapsid gene (e.g., N1, N2) as well as the endogenous indicator pepper mild mottle virus (PMMoV) were quantified using RT-qPCR. Our study demonstrated that cotton gauze was able to identify trends in SARS-CoV-2 burdens on campus and that viral concentrations on passive samplers were significantly correlated with known clinical cases in the catchment. The dataset also includes monitoring data collected at the nearby municipal WWTP which offers insight to transmission dynamics at both sampling scales. Further developing affordable WBS methodologies will benefit future monitoring efforts under novel public health scenarios.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution 4.0 International (CC BY 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
en_US
dc.subject
SARS-CoV-2
en_US
dc.subject
Omicron
en_US
dc.subject
wastewater
en_US
dc.subject
passive sampling
en_US
dc.subject
wastewater-based surveillance
en_US
dc.title
Implementing an adaptive, two-tiered SARS-CoV-2 wastewater surveillance program on a university campus using passive sampling
en_US
globus.shared_endpoint.name
9347409e-b290-40c8-9b35-8bc3bae5d046
globus.shared_endpoint.path
/2/published/publication_851/
datacite.publicationyear
2023
datacite.contributor.DataCollector
Blake R. Haskell
datacite.contributor.DataManager
Hadi A. Dhiyebi
datacite.contributor.Supervisor
Mark Servos
datacite.date.Collected
2021-08-18/2022-04-29
datacite.resourcetype
Dataset
en_US
datacite.relatedidentifier.IsPartOf
https://doi.org/10.1016/j.scitotenv.2023.168998
datacite.geolocation.geolocationPoint
43.470754 -80.542594
datacite.geolocation.geolocationPlace
University of Waterloo;Waterloo;Ontario;Canada
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
Ontario Ministry of the Environment, Conservation and Parks
en_US
datacite.fundingReference.awardNumber
TPA 2021-02-1-1564736554
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
Canada First Research Excellence Fund (CFREF)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
Global Water Futures Program
en_US
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada
en_US
datacite.fundingReference.awardNumber
RGPIN-2017-03816
en_US
datacite.fundingReference.awardTitle
Discovery
en_US
datacite.fundingReference.funderIdentifier
https://ror.org/0517h6h17
en_US
datacite.fundingReference.funderName
Canada Research Chairs
en_US
datacite.fundingReference.awardNumber
CRC-2017-00230
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
Global Water Futures
en_US
datacite.fundingReference.awardNumber
410295
en_US
datacite.fundingReference.awardTitle
Lake Futures: Enhancing Adaptive Capacity and Resilience of Lakes and their Watersheds
en_US
frdr.crdc.code
RDF1069999
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Other biological sciences
en_US
frdr.crdc.field_en
Other biological sciences
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Autres sciences biologiques
fr_CA
frdr.crdc.field_fr
Autres sciences biologiques
fr_CA
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