Field
Value
Language
dc.contributor.author
Ding, Yuzhe
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Zhang, Ziyu
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Kaiyum, Yunus
datacite.creator.affiliationIdentifier
https://ror.org/05fq50484
en_US
datacite.creator.affiliation
York University
en_US
datacite.creator.nameIdentifier
https://orcid.org/0009-0001-3086-9124
en_US
dc.contributor.author
Heng, Yicheng
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Johnson, Philip
datacite.creator.affiliationIdentifier
https://ror.org/05fq50484
en_US
datacite.creator.affiliation
York University
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0002-5573-4891
en_US
dc.contributor.author
Liu, Juewen
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.date.accessioned
2025-01-15T17:25:42Z
dc.date.available
2025-01-15T17:25:42Z
dc.date.issued
2025-01-15
dc.identifier.uri
https://doi.org/10.20383/103.01054
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/14c728a0-1b72-48dc-bc6a-f00c393f451b
dc.description
During a typical aptamer selection experiment, buffer molecules are used at 10 to 50 mM, whereas target molecules could be used at much lower concentrations even in low µM levels. Therefore, doubts existed regarding the potential enrichment of buffer binding aptamers, particularly for failed selections that cannot validate binding of enriched sequences. In this study, we respectively used two common buffer molecules, Tris and HEPES, as target molecules. While we successfully isolated aptamers for Tris buffer, our attempts to generate aptamers for HEPES buffer failed. Thioflavin T (ThT) fluorescence spectroscopy confirmed the dissociation constant of the Tris buffer aptamer to be 2.9 mM, while isothermal titration calorimitry showed a Kd of 43 µM. NMR spectroscopy also confirmed aptamer binding. Finally, we discussed the implications of this buffer selection work and recommended the use of certain buffers.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution 4.0 International (CC BY 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
en_US
dc.subject
DNA
en_US
dc.subject
SELEX
en_US
dc.subject
Aptamer
en_US
dc.subject
Tris
en_US
dc.subject
HEPES
en_US
dc.title
DNA aptamers for common buffer molecules: possibility of buffer interference in SELEX
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_1049/
datacite.publicationYear
2025
datacite.date.Collected
2023-08-09/2024-08-30
datacite.resourceType
Dataset
en_US
datacite.relatedIdentifier.IsCitedBy
https://doi.org/10.1039/D4OB00622D
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1060101
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
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