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Differential sex responses of fathead minnow (Pimephales promelas) gut microbiota to a short-term environmentally-relevant aqueous exposure to benzo[a]pyrene

Description: Vertebrate gut microbiota are responsible for regulating several beneficial functions, from development of an organism to maintaining energy homeostasis. However, little is known about the impact of chemical exposures on the structure and function of gut microbiota of fishes. To ascertain a link between contaminant exposure and microbial disruption, male and female fathead minnows (Pimephales promelas) were aqueously exposed to low concentrations of benzo[a]pyrene (BaP) (concentrations ranged from 0.0226 g/L to 1.3 g/L) as well as fish exposed to a solvent control for four days. The samples were sterilely collected from whole fathead minnow guts and stored at -80C until DNA extraction. Gut microbiota were assessed using 16S rRNA metagenetics. Low-level aqueous exposure to BaP resulted in community shifts in bacterial composition in female, but not male, fish. Each fish was analyzed independently (samples were not pooled).This research illustrates that in addition to the well-studied molecular endpoints, community composition of fish gut microbiota can also be impacted by chemical stressors, providing an additional pathway for the generation of adverse effects.
Notes: Item exited embargo and became publicly available on 2020-07-01
L’élément est sorti de sa période d’embargo et est devenu public le 2020-07-01
Authors: DeBofsky, Abigail; University of Saskatchewan; Toxicology Centre; https://orcid.org/0000-0003-3877-6421 ORCID iD
Xie, Yuwei; University of Saskatchewan; Toxicology Centre; https://orcid.org/0000-0001-5652-6413 ORCID iD
Grimard, Chelsea; University of Saskatchewan; Toxicology Centre
Brinkmann, Markus; University of Saskatchewan; Toxicology Centre; https://orcid.org/0000-0002-4985-263 ORCID iD
Hecker, Markus; University of Saskatchewan; Toxicology Centre; https://orcid.org/0000-0002-7237-6192 ORCID iD
Giesy, John; University of Saskatchewan; Toxicology Centre; https://orcid.org/0000-0003-1931-9962 ORCID iD
Keywords: microbiome
16S
sequencing
PAHs
microbial community
fish
freshwater
risk assessment
fish health
fathead minnow
Pimephales promelas
Date: 1-Jul-2020
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
URI: https://doi.org/10.20383/101.0196
Related Identifiers: 
Geographic Coverage: 
Place Name
Aquatic Toxicology Research Facility - University of Saskatchewan Toxicology Centre
City
Saskatoon
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada
Geographic Point: 
Latitude
52.133749
Longitude
-106.631895
Appears in Collections:Global Water Futures

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Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
DeBofsky, A. , Xie, Y. , Grimard, C. , Brinkmann, M. , Hecker, M. , Giesy, J. (2020) Differential sex responses of fathead minnow (Pimephales promelas) gut microbiota to a short-term environmentally-relevant aqueous exposure to benzo[a]pyrene. Federated Research Data Repository. https://doi.org/10.20383/101.0196