Field
Value
Language
dc.contributor.author
Huang, Po-Jung Jimmy
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-3436-9968
en_US
dc.contributor.author
Li, Albert Zehan
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0009-0008-6294-5073
en_US
dc.contributor.author
Dieckmann, Thorsten
en_US
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Liu, Juewen
en_US
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.date.accessioned
2023-10-20T19:43:36Z
dc.date.available
2023-10-20T19:43:36Z
dc.date.issued
2023-10-20
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/1b1aaa1a-36b2-458a-b0b9-c82bf23da210
dc.identifier.uri
https://doi.org/10.20383/103.0826
dc.description
We recently reported that some adenosine binding aptamers can also bind caffeine and theophylline with around 20-fold lower affinities. This discovery led to the current work to examine the cross-binding of adenosine to theophylline aptamers. For the DNA aptamer for theophylline, cross-binding to adenosine was observed, and the affinity was 18 to 38-fold lower for adenosine based on assays using isothermal titration calorimetry and ThT fluorescence spectroscopy. The binding complexes were characterized using NMR spectroscopy, and both adenosine and theophylline showed an overall similar binding structure to the DNA theophylline aptamer, although small differences were also observed. In contrast, the RNA aptamer did not show binding to adenosine, although both aptamers have very similar relative selectivity for various methylxanthines including caffeine. After a negative selection, a few new aptamers with completely different primary sequences for theophylline were obtained and they did not show binding to adenosine. Thus, there are many ways for aptamers to bind theophylline and some can have cross-binding to adenosine. In biology, theophylline, caffeine, and adenosine can bind to the same protein receptors to regulate sleep, and their binding to the same DNA motifs may suggest an early role of nucleic acids in similar regulatory functions.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution 4.0 International (CC BY 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
en_US
dc.subject
DNA
en_US
dc.subject
SELEX
en_US
dc.subject
Aptamer
en_US
dc.subject
Adenosine
en_US
dc.subject
Theophylline
en_US
dc.title
Cross-Binding of Adenosine by Aptamers Selected Using Theophylline
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_821/
datacite.publicationYear
2023
datacite.contributor.DataCollector
Natasha M. Evans
datacite.contributor.DataCollector
Chang Lu
datacite.contributor.DataCollector
Albert Zehan Li
datacite.contributor.ProjectManager
Po-Jung Jimmy Huang
datacite.contributor.Researcher
Po-Jung Jimmy Huang
en_US
datacite.contributor.ResearchGroup
Thorsten Dieckmann
datacite.contributor.ResearchGroup
Juewen Liu
datacite.date.Collected
2021-12-01/2023-06-01
en_US
datacite.resourceType
Dataset
en_US
datacite.relatedIdentifier.IsCitedBy
https://doi.org/10.1002/cbic.202300566
datacite.geolocation.geolocationPlace
University of Waterloo;Waterloo;Ontario;Canada
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
https://ror.org/02tvrwm90
en_US
datacite.fundingReference.funderName
Canada Excellence Research Chairs
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
Global Water Future project
en_US
frdr.crdc.code
RDF1060101
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
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