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Responses of juvenile fathead minnow (Pimephales promelas) gut microbiota to a chronic dietary exposure of benzo[a]pyrene

Description: These data are collected from juvenile fathead minnows (Pimephales promelas) that were exposed to benzo[a]pyrene (BaP) as well as fish exposed to a solvent control for two weeks via the diet. The samples were sterilely collected from whole fathead minnow guts and stored at -80°C until DNA and RNA extraction. The guts were used for 16S rRNA sequencing to analyze the impact of BaP on gut microbial communities. Each fish was analyzed independently (samples were not pooled). The results of this study showed a significant effect of BaP exposure on gut microbial community.
Notes: Item exited embargo and became publicly available on 2020-07-01
Authors: DeBofsky, Abigail; Toxicology Centre; University of Saskatchewan; ORCID iD 0000-0003-3877-6421
Xie, Yuwei; Toxicology Centre; University of Saskatchewan; ORCID iD 0000-0001-5652-6413
Challis, Jonathan; Toxicology Centre; University of Saskatchewan
Brinkmann, Markus; Toxicology Centre; University of Saskatchewan; ORCID iD 0000-0002-4985-263
Hecker, Markus; Toxicology Centre; University of Saskatchewan; ORCID iD 0000-0002-7237-6192
Giesy, John; Toxicology Centre; University of Saskatchewan; ORCID iD 0000-0003-1931-9962
Keywords: Pimephales promelas
16S rRNA sequencing
Polycyclic aromatic hydrocarbons
PAHs
Microbial community
Fish
Freshwater
Risk assessment
Fish health
Field of Research: 
Biological sciences
>
Microbiology
>
Microbiome
Publication Date: 2020-07-01
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Global Water Futures (GWF); Next generation solutions to ensure healthy water resources for future generations
Canada First Research Excellence Fund (CFREF); Global Water Futures Program (GWF); 419205
URI: https://doi.org/10.20383/101.0247
Related Identifiers: 
Geographic Coverage: 
Place Name
University of Saskatchewan - Aquatic Toxicology Research Facility (ATRF)
City
Saskatoon
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada
Geographic Point: 
Latitude
52.133749
Longitude
-16.631895
Appears in Collections:Global Water Futures

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
DeBofsky, A., Xie, Y., Challis, J., Brinkmann, M., Hecker, M., Giesy, J. (2020). Responses of juvenile fathead minnow (Pimephales promelas) gut microbiota to a chronic dietary exposure of benzo[a]pyrene. Federated Research Data Repository. https://doi.org/10.20383/101.0247