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Sahu et al. STORM Data
Description: | Sodium and potassium channels that regulate axonal spike propagation are highly organized at nodes of Ranvier by a spectrin-actin membrane periodic skeleton. STORM-TIRF microscopy was used to define the spatial organization over the soma of a complex of Cav1.3 calcium, RyR2, and IK potassium channels (CaRyK) that generate a slow AHP in hippocampal neurons. Nearest neighbor distance and non-negative matrix factorization analyses identified two spatial patterns as linear rows of 3-8 immunolabeled clusters with 155 nm periodicity that extended to branchpoints, or as isolated clusters with 600-800 nm separation. The same patterns were detected for spectrin βII and the actin linking proteins actinin I and II that substantially overlapped with CaRyK complex clusters. The data reveal a close correspondence between CaRyK complex proteins and a net-like structure of spectrin βII across the soma that coordinates the spatial organization of channels that control the pattern of spike output. |
Notes: | Item exited embargo and became publicly available on 2024-04-24 |
Authors: | Sahu, Giriraj; Hotchkiss Brain Institute, Cummings School of Medicine, University of Calgary; University of Calgary; 0000-0001-6566-9563 Greening, Dylan; Hotchkiss Brain Institiute, Cumming School of Medicine, University of Calgary; Department of Clinical Neuroscience, Cumming School of Medicine, University of Calgary; University of Calgary; 0009-0006-1756-571X Wilten, Nicola; Department of Cell Biology & Anatomy, Cumming School of Medicine, University of Calgary; Hotchkiss Brain Institiute, Cumming School of Medicine, University of Calgary; University of Calgary Turner, Raymond W; Hotchkiss Brain Institiute, Cumming School of Medicine, University of Calgary; Department of Clinical Neuroscience, Cumming School of Medicine, University of Calgary; University of Calgary |
Keywords: | CaV1.3 KCa3.1 RyR2 spectrin cytoskeleton ER-PM junction slow AHP hippocampus |
Field of Research: | Basic medicine and life sciences > Neurosciences, medical and physiological and health aspects > Neurosciences, medical and health and physiological aspects, not elsewhere classified
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Publication Date: | 2024-04-24 |
Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
Funder: | Canadian institute of health research; The molecular choreography of ion channel expression (RWT); Ref# PJT-169007 Natural Sciences and Engineering Research Council of Canada; Discovery grant |
URI: | https://doi.org/10.20383/103.0809 |
Geographic Coverage: | City Calgary Province / Province / Territory Alberta Territory Country Canada |
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Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Sahu, G., Greening, D., Wilten, N., Turner, R. (2024). Sahu et al. STORM Data. Federated Research Data Repository. https://doi.org/10.20383/103.0809