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Characterization of high mountain glacier microbiota following inundation with wildfire soot

Description: Glacier ecosystems are shrinking at an accelerating rate due to changes in climate, but also due to increased deposition of soot, which has resulted in darkening of glacier surfaces and subsequent changes in absorption of light, associated heat, and altered microbial communities. In this study, compositions of microbial communities on surfaces of the Athabasca Glacier (Alberta, Canada) were measured by use of DNA metabarcoding. Three matrices, glacier ice, cryoconite hole, and glacial surface sediment, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata.
Notes: Item exited embargo and became publicly available on 2022-01-01
L’élément est sorti de sa période d’embargo et est devenu public le 2022-01-01
Authors: Esser, Milena; University of Saskatchewan; https://orcid.org/0000-0002-2802-6160 ORCID iD
Ankley, Phillip; University of Saskatchewan; https://orcid.org/0000-0002-5883-6148 ORCID iD
Aubry-Wake, Caroline; University of Saskatchewan; https://orcid.org/ 0000-0002-9452-8580 ORCID iD
Xie, Yuwei; University of Saskatchewan; https://orcid.org/0000-0001-5652-6413 ORCID iD
Baulch, Helen; University of Saskatchewan; https://orcid.org/ 0000-0001-9018-4998 ORCID iD
Hoggarth, Cameron; University of Saskatchewan
Hecker, Markus; University of Saskatchewan; https://orcid.org/0000-0002-7237-6192 ORCID iD
Hollert, Henner; Goethe University Frankfurt; https://orcid.org/ 0000-0001-5776-5619 ORCID iD
Giesy, John; University of Saskatchewan; Baylor University; https://orcid.org/0000-0003-1931-9962 ORCID iD
Pomeroy, John; University of Saskatchewan; https://orcid.org/0000-0002-4782-7457 ORCID iD
Brinkmann, Markus; University of Saskatchewan; https://orcid.org/ 0000-0002-4985-263X ORCID iD
Keywords: Athabasca Glacier
ecosystem
microbiome
soot
deposition
PAHs
Field of Research: 
Biological sciences
>
Microbiology
>
Microbiome
Date: 1-Jan-2022
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
URI: https://doi.org/10.20383/102.0528
Geographic Coverage: 
Place Name
Athabasca Glacier
Province /
Province / Territory
Alberta
Territory
 
Country
Canada
Geographic Point: 
Latitude
52.19182
Longitude
-117.25165
Appears in Collections:Global Water Futures

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Esser, M. , Ankley, P. , Aubry-Wake, C. , Xie, Y. , Baulch, H. , Hoggarth, C. , Hecker, M. , Hollert, H. , Giesy, J. , Pomeroy, J. , Brinkmann, M. (2022) Characterization of high mountain glacier microbiota following inundation with wildfire soot. Federated Research Data Repository. https://doi.org/10.20383/102.0528