Field
Value
Language
dc.contributor.author
Huang, Po-Jung Jimmy
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-3436-9968
en_US
dc.contributor.author
Liu, Juewen
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.coverage.temporal
2020-03-31/2021-04-30
dc.date.accessioned
2022-06-10T17:06:58Z
dc.date.available
2022-06-10T17:06:58Z
dc.date.issued
2022-06-10
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/53e306d1-496d-4fb9-9fd5-985751d6405f
dc.identifier.uri
https://doi.org/10.20383/103.0587
dc.description
With the growing consumption of caffeine-containing beverages, detection of caffeine has become an important biomedical, bioanalytical, and environmental topic. Four high-quality aptamers for caffeine were isolated and were carefully characterized. A structure-switching fluorescent sensor was designed showing a detection limit of 1.2 μM caffeine, which reflected the labeled caffeine concentration within 6.1% difference for eight commercial beverages. In 20% human serum, a detection limit of 4.0 μM caffeine was achieved. In addition, these four fluorescent aptamer sensors can form a sensing array, caffeine and the three analogues were well separated from nine other closely related molecules.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
DNA
en_US
dc.subject
SELEX
en_US
dc.subject
fluorescence
en_US
dc.subject
Aptamer
en_US
dc.subject
Caffeine
en_US
dc.subject
Biosensor
en_US
dc.title
Selection of Aptamers for Sensing Caffeine and Discrimination of Its Three Single Demethylated Analogues
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_582/
frdr.preservation.status
AIP generation and transfer successful.
frdr.preservation.datetime
2023-11-03
datacite.publicationyear
2022
datacite.resourcetype
Dataset
en_US
datacite.relatedidentifier.IsCitedBy
https://doi.org/10.1021/acs.analchem.1c04349
datacite.geolocation.geolocationPlace
University of Waterloo;Waterloo;Ontario;Canada
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada (NSERC)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
Canada First Research Excellence Fund
en_US
frdr.crdc.code
RDF1060101
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
datacite.description.other
Dataset is from the publication "Selection of Aptamers for Sensing Caffeine and Discrimination of Its Three Single Demethylated Analogues"
https://doi.org/10.1021/acs.analchem.1c04349
datacite.description.other
Item exited embargo and became publicly available on 2022-06-10
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