Field
Value
Language
dc.contributor.author
Zandieh, Mohamad
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-9560-6184
en_US
dc.contributor.author
Liu, Juewen
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.date.accessioned
2023-08-17T13:40:13Z
dc.date.available
2023-08-17T13:40:13Z
dc.date.issued
2023-08-17
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/64573292-0ec8-401f-a429-4e55ce58ec50
dc.identifier.uri
https://doi.org/10.20383/103.0773
dc.description
Nanodiamonds (NDs) have attracted considerable attention owing to their quantum properties and versatility in biological applications. In this study, we systematically investigated the adsorption of DNA oligonucleotides onto NDs with three types of surface groups: carboxylated (COOH-), hydroxylated (OH-), and hydrogenated (H-). Among them, only the H-NDs showed fluorescence quenching property that is useful for real-time DNA adsorption kinetic studies. The effect of common metal ions on DNA adsorption was studied. In the presence of Na+, the order of DNA adsorption efficiency was H-> OH- > COOH-, whereas all the NDs showed a similar DNA adsorption efficiency in the presence of divalent metal ions such as Ca2+ and Zn2+. Desorption studies revealed that hydrogen bonding and metal-mediated interactions were dominant for the adsorption of DNA, and the H-NDs exhibited extraordinarily tight DNA adsorption. Finally, a fluorescently labeled DNA was adsorbed on NDs for DNA detection, and the COOH-NDs had the highest target specificity, and a detection limit of 1.4 nM was achieved. This study indicates the feasibility of using metal ions to mediate the physical adsorption of DNA to NDs and compares various NDs with graphene oxide for fundamental understanding.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
Nanodiamonds
en_US
dc.subject
DNA
en_US
dc.subject
Oligonucleotides
en_US
dc.subject
Adsorption
en_US
dc.subject
Surface Science
en_US
dc.title
Metal-Mediated DNA Adsorption on Carboxylated, Hydroxylated and Hydrogenated Nanodiamonds
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_768/
datacite.publicationYear
2023
datacite.date.Collected
2023-01-01/2023-05-01
datacite.resourceType
Dataset
en_US
datacite.relatedIdentifier.IsCitedBy
https://doi.org/10.1021/acs.langmuir.3c01066
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
Global Water Futures
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1040106
en_US
frdr.crdc.group_en
Chemical sciences
en_US
frdr.crdc.class_en
Analytical chemistry
en_US
frdr.crdc.field_en
Sensor technology (chemical aspects)
en_US
frdr.crdc.group_fr
Sciences chimiques
fr_CA
frdr.crdc.class_fr
Chimie analytique
fr_CA
frdr.crdc.field_fr
Technologie des capteurs (aspects chimiques)
fr_CA
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