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DNA and RNA zooplankton metabarcoding to assess the efficacy of different oil spill clean-up techniques in a boreal lake

Description: Emerging tools, namely metabarcoding, has promise for high-throughput and benchmarkable biomonitoring of freshwater zooplankton communities. Additionally, regulators require further information to select best practices for remediating freshwater ecosystems after oil spills. DNA and RNA metabarcoding, or present and active zooplankton, respectively, was applied to compare with traditional morphological identification of freshwater zooplankton in experimental boreal shoreline enclosures. DNA and RNA metabarcoding was also applied in the context of assessing response of the zooplankton community exposed to simulated spills of diluted bitumen (dilbit), with experimental remediation practices of enhanced monitored natural recovery and shoreline cleaner application. Zooplankton samples were collected via pump on day -3 and 11 and 38 days after the simulated dilbit spill. The zooplankton samples were co-extracted for DNA and RNA and were PCR amplified targeting the mitochondrial Cytochrome c Oxidase subunit I gene (CO1) region, with amplicon sequencing following. This dataset includes the demultiplexed sequencing output, the feature table with genus-level taxonomic annotation, and the sample metadata used for hypothesis testing. This dataset contains data from wetland habitat enclosures. Note that a similar study was conducted for rock habitat enclosures, with different analyses and interpretation being conducted on the data (dataset available at https://doi.org/10.20383/102.0332).
Notes: Item exited embargo and became publicly available on 2021-06-01
L’élément est sorti de sa période d’embargo et est devenu public le 2021-06-01
Authors: Ankley, Phillip; University of Saskatchewan; https://orcid.org/0000-0002-5883-6148 ORCID iD
Xie, Yuwei; University of Saskatchewan; https://orcid.org/0000-0001-5652-6413 ORCID iD
Black, Tyler; University of Guelph
DeBofsky, Abigail; University of Saskatchewan; https://orcid.org/0000-0003-3877-6421 ORCID iD
Perry, McKenzie; University of Manitoba
Paterson, Michael; International Institute for Sustainable Development
Hanson, Mark; University of Manitoba
Higgins, Scott; International Institute for Sustainable Development
Giesy, John; University of Saskatchewan; https://orcid.org/0000-0003-1931-9962 ORCID iD
Palace, Vince; International Institute for Sustainable Development
Keywords: metabarcoding
zooplankton
mitochondrial CO1
next-generation sequencing
diluted bitumen
oil-spill
remediation
Boreal
Canada
freshwater
ecotoxicology
Date: 1-Jun-2021
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
URI: https://doi.org/10.20383/101.0313
Related Identifiers: 
Geographic Coverage: 
Place Name
International Institute for Sustainable Development – Experimental Lakes Area
Province /
Province / Territory
Ontario
Territory
 
Country
Canada
Geographic Point: 
Latitude
49.6964
Longitude
-93.76752
Appears in Collections:Global Water Futures

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Ankley, P. , Xie, Y. , Black, T. , DeBofsky, A. , Perry, M. , Paterson, M. , Hanson, M. , Higgins, S. , Giesy, J. , Palace, V. (2021) DNA and RNA zooplankton metabarcoding to assess the efficacy of different oil spill clean-up techniques in a boreal lake. Federated Research Data Repository. https://doi.org/10.20383/101.0313