Field
Value
Language
dc.contributor.author
Lide, Gu
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian, China
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Hanxiao, Zhang
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON, Canada
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Yuzhe, Ding
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON, Canada
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Yao, Zhang
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian, China
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Deli, Wang
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian, China
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Juewen, Liu
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, ON, Canada
en_US
datacite.creator.nameIdentifier
en_US
dc.date.accessioned
2023-08-22T17:31:01Z
dc.date.available
2023-08-22T17:31:01Z
dc.date.issued
2023-08-22
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/a565b8bc-308f-476c-8c63-054d5f0b5913
dc.identifier.uri
https://doi.org/10.20383/103.0785
dc.description
Salicylic acid (SA) is the hydrolysis product and active form of aspirin, and SA is found in a range of fruits and other food products. For food and drug chemistry and analysis, there is a strong desire to detect SA. Since SA is a very small molecule, aptamers have advantages for the detection of SA. In this work, we used the library-immobilization capture-SELEX method to isolate aptamers for SA. After 17 rounds of selection, two main families of aptamers were isolated. The SA1 aptamer from family 1 has a Kd of 5.8 µM from a thioflavin T (ThT) fluorescence assay and 26.7µM from isothermal titration calorimetry (ITC). The binding of other sequence was weaker compared to SA1. Based on mutation studies, the two conserved regions of SA1 were connected by two stems. Using ThT, a fluorescent sensor was tested for the detection of SA with a detection limit of 2.2 µM. A range of similar molecules were tested including aspirin, and only p-hydroxybenzoic acid showed a weak binding to the SA1 aptamer, indicating the high specificity of the SA1 aptamer. Finally, the SA1 aptamer was also tested in tomato juice and a similar binding performance was achieved
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
DNA
en_US
dc.subject
aptamer
en_US
dc.subject
selex
en_US
dc.subject
Salicylic acid
en_US
dc.title
Capture-SELEX for a short aptamer for label-free detection of salicylic acid
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_780/
datacite.publicationYear
2023
datacite.resourceType
Dataset
en_US
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
https://ror.org/04atp4p48
en_US
datacite.fundingReference.funderName
China Scholarship Council
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1060101
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
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