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Enzymatic degradation of polyethylene terephthalate (PET) using Hic cutinase
| Description: | This dataset contains data collected on enzymatic degradation of PET caused by Huimcola insolens cutinase. This includes differences in degradation by varying temperatures which included incubation at 25 ˚C, 40 ˚C and 55 ˚C; pretreatments which included freeze-thaw cycles (FTC) and exposure to extreme temperatures, -70 ˚C or +55 ˚C; and plastic type, assessing the differences between laboratory grade PET and consumer grade plastic water bottle PET. Analyses conducted included mass loss, Fourier-transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) to determine crystallinity, scanning electron microscopy (SEM) images for select samples, thermo gravimetric analysis (TGA), tensile strength and dissolved organic carbon (DOC) to confirm degradation. Mass loss rates for each treatment are reported. |
| Authors: | Griffiths, Erin; University of Waterloo;  0009-0002-5435-4392Slowinski, Stephanie; University of Waterloo; 0000-0001-5090-441XHonek, John; University of Waterloo; 0000-0002-4035-4538Smith, Rodney; University of Waterloo; 0000-0003-1209-9653Reza Nezhad, Fereidoun; University of Waterloo; 0000-0002-9608-8005Van Cappellen, Philippe; University of Waterloo; 0000-0001-5476-0820 |
| Keywords: | Polyethylene terephthalate enzymatic degradation hydrolysis enzyme Humicola insolens cutinase microplastics |
| Field of Research: | Earth and related environmental sciences > Other earth and related environmental sciences > Earth system sciences
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| Publication Date: | 2025-03-14 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council of Canada; Microplastics Fingerprinting at the watershed scale: from sources to receivers; ALLRP 558435-20 Canada First Research Excellence Fund; Global Water Futures |
| URI: | https://doi.org/10.20383/103.0897 |
| Geographic Coverage: | City Waterloo Province / Province / Territory Ontario Territory Country Canada |
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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Griffiths, E., Slowinski, S., Honek, J., Smith, R., Reza Nezhad, F., Van Cappellen, P. (2025). Enzymatic degradation of polyethylene terephthalate (PET) using Hic cutinase. Federated Research Data Repository. https://doi.org/10.20383/103.0897
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