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Enzymatic degradation of polyethylene terephthalate (PET) using Hic cutinase

Description: This dataset contains data collected on enzymatic degradation of PET caused by Huimcola insolens cutinase. This includes differences in degradation by varying temperatures which included incubation at 25 ˚C, 40 ˚C and 55 ˚C; pretreatments which included freeze-thaw cycles (FTC) and exposure to extreme temperatures, -70 ˚C or +55 ˚C; and plastic type, assessing the differences between laboratory grade PET and consumer grade plastic water bottle PET. Analyses conducted included mass loss, Fourier-transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) to determine crystallinity, scanning electron microscopy (SEM) images for select samples, thermo gravimetric analysis (TGA), tensile strength and dissolved organic carbon (DOC) to confirm degradation. Mass loss rates for each treatment are reported.
Authors: Griffiths, Erin; University of Waterloo; ORCID iD 0009-0002-5435-4392
Slowinski, Stephanie; University of Waterloo; ORCID iD 0000-0001-5090-441X
Honek, John; University of Waterloo; ORCID iD 0000-0002-4035-4538
Smith, Rodney; University of Waterloo; ORCID iD 0000-0003-1209-9653
Reza Nezhad, Fereidoun; University of Waterloo; ORCID iD 0000-0002-9608-8005
Van Cappellen, Philippe; University of Waterloo; ORCID iD 0000-0001-5476-0820
Keywords: Polyethylene terephthalate
enzymatic degradation
hydrolysis enzyme
Humicola insolens cutinase
Field of Research: 
Earth and related environmental sciences
Other earth and related environmental sciences
Earth system sciences
Publication Date: 2025-03-14
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Natural Sciences and Engineering Research Council; Microplastics Fingerprinting at the watershed scale: from sources to receivers; ALLRP 558435-20
Canada First Research Excellence Fund; Global Water Futures
Geographic Coverage: 
Province /
Province / Territory

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0)
Griffiths, E., Slowinski, S., Honek, J., Smith, R., Reza Nezhad, F., Van Cappellen, P. (2025). Enzymatic degradation of polyethylene terephthalate (PET) using Hic cutinase. Federated Research Data Repository.