Field
Value
Language
dc.contributor.author
Huang, Po-Jung Jimmy
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-3436-9968
en_US
dc.contributor.author
Liu, Juewen
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.date.accessioned
2023-04-25T12:37:22Z
dc.date.available
2023-04-25T12:37:22Z
dc.date.issued
2023-04-25
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/bd6a1fca-eb2f-4ee1-9310-2cec4f6a7780
dc.identifier.uri
https://doi.org/10.20383/102.0714
dc.description
Since the report of the RNA aptamer for theophylline, theophylline has become a key molecule in chemical biology for designing RNA switches and riboswitches. In addition, theophylline is an important drug for treating airway diseases including asthma. The classic RNA aptamer with excellent selectivity for theophylline has been used to design biosensors, although DNA aptamers are more desirable for stability and cost considerations. In this work, we selected DNA aptamers for theophylline, and all the top sequences shared the same binding motifs. Binding was confirmed using isothermal titration calorimetry and a nuclease digestion assay, showing a dissociation constant (Kd) around 0.5 μM theophylline. The Theo2201 aptamer can be truncated down to 23-mer while still has a Kd of 9.8 μM. The selectivity for theophylline over caffeine is around 250,000-fold based on a strand-displacement assay, which was more than 20-fold higher compared to the classic RNA aptamer. For other tested analogs, the DNA aptamer also showed better selectivity. Using the structure-switching aptamer sensor design method, a detection limit of 17 nM theophylline was achieved in the selection buffer, and a detection limit of 31 nM was obtained in 10% serum.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
DNA
en_US
dc.subject
SELEX
en_US
dc.subject
Aptamer
en_US
dc.subject
Theophylline
en_US
dc.subject
Biosensor
en_US
dc.subject
RNA
en_US
dc.title
A DNA Aptamer for Theophylline with Ultrahigh Selectivity Reminiscent of the Classic RNA Aptamer
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/2/published/publication_709/
datacite.publicationyear
2023
datacite.date.Collected
2020-09-01/2021-03-01
datacite.resourcetype
Dataset
en_US
datacite.relatedidentifier.IsCitedBy
https://doi.org/10.1021/acschembio.2c00179
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada (NSERC)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
Canada First Research Excellence Fund (CFREF)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
Global Water Futures Program (GWF)
en_US
frdr.crdc.code
RDF1060101
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
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