Field
Value
Language
dc.contributor.author
Azam, Iqra
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Olver, Dominic
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-1580-5023
en_US
dc.contributor.author
Benson, James
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-1819-1899
en_US
dc.coverage.temporal
2020-10-01/2023-12-15
dc.date.accessioned
2023-11-15T19:31:44Z
dc.date.available
2023-11-15T19:31:44Z
dc.date.issued
2023-11-15
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/d46b8b2c-5fc3-41b1-b247-8cd6c4c3f837
dc.identifier.uri
https://doi.org/10.20383/103.0843
dc.description
We investigated the equilibrium and dynamic cell and nuclear volumetric responses in adherent human hepatoblastoma (HepG2) cell monolayers using live three-dimensional imaging. We have further demonstrated that both cells and nuclei regulate their volume in response to osmotic stress. For instance, during hyposmotic stress, cells swell and lose important osmolytes through active volume regulation or bleb formation, however, nuclei undergo more expansion than cells. Whereas during hyperosmotic stress, both cells and nuclei shrink rapidly into irregular shapes and undergo passive volume regulation. Furthermore, we explored the mechanism of osmoregulation in these cells by restoring isosmotic conditions after osmotic stress and found a higher return-to-equilibrium volume for nuclei compared to their initial isosmotic volume, while cytosol returned to a lower volume than its isosmotic volume after swelling. Moreover, water and solute permeability estimates for both cells and nuclei were also inferred from exposure to different anisosmotic media by fitting coupled transport models. This accurate estimation of cell and nuclear volumetric responses and water and solute permeability parameters could have important implications for understanding cell physiology and the mechanism of osmoregulation.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
en_US
dc.subject
Cell volume
en_US
dc.subject
Osmotic stress
en_US
dc.subject
Three-dimensional imaging
en_US
dc.subject
Image analysis
en_US
dc.subject
Nuclear biophysical parameters
en_US
dc.title
4D confocal microscopy of time dependent cell and nuclear osmotic response reveals differential osmoregulation and transport kinetics
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/1/published/publication_838/
datacite.publicationyear
2023
datacite.contributor.DataCollector
Iqra Azam
datacite.date.Collected
2021-02-01/2023-12-15
datacite.resourcetype
Dataset
en_US
datacite.geolocation.geolocationPoint
52.132854 -106.631401
datacite.geolocation.geolocationPlace
University of Saskatchewan;Saskatoon;Saskatchewan;Canada
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
National Science and Engineering Research Council
en_US
datacite.fundingReference.awardNumber
RGPIN-2023-04007
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1060299
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Cell biology
en_US
frdr.crdc.field_en
Cell biology, not elsewhere classified
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biologie cellulaire
fr_CA
frdr.crdc.field_fr
Biologie cellulaire, non classé ailleurs
fr_CA