How to Download
FRDR offers multiple ways to download datasets. Learn more in our documentation.
Capture-SELEX of DNA Aptamers for Sulforhodamine B and Fluorescein
| Description: | While many dye binding aptamers have been reported, most of them were for light-up aptamers that can significantly enhance the quantum yield of fluorophores. Sulforhodamine B (SRhB) was used as a target previously to select both DNA and RNA aptamers, and the DNA aptamer was a G-quadruplex that can bind to a number of rhodamine analogs. In addition, the previous selections were performed by immobilizing the target molecules. In this work, we used the library immobilization method to respectively select aptamers for SRhB and fluorescein. The SRhB aptamer has a non-G-quadruplex structure with a Kd of 1.0 µM measured from isothermal titration calorimetry. Upon titration of the aptamer, the fluorescence of SRhB increased 2.5-fold, and this aptamer does not require Mg2+ for binding. Rhodamine B has even tighter binding suggesting binding through the xanthene moiety of the dyes. No binding was detected for fluorescein. For the fluorescein selection, a dominant aptamer sequence with a Kd of 147 µM was obtained. This study provides two new aptamers for two important fluorophores that can be used to study aptamer-based separation, dye detection, and catalysis. Comparison of these aptamers also provides insights into the effect of functional groups on aptamer binding. |
| Authors: | Lide, Gu; Xiamen University; University of Waterloo;  0000-0002-9466-9674Jiajie, Zheng; University of Waterloo Yao, Zhang; Xiamen University Deli, Wang; Xiamen University Juewen, Liu; University of Waterloo; 0000-0001-5918-9336 |
| Keywords: | Aptamers Biosensors Fluorescein Sulforhodamine B SELEX |
| Field of Research: | Biological sciences > Biochemistry > Analytical biochemistry
|
| Publication Date: | 2023-09-25 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council of Canada (NSERC) |
| URI: | https://doi.org/10.20383/103.0808 |
Files in Dataset
No files uploaded
Download entire dataset using Globus Transfer. This method requires a Globus account and installing software. Watch Video: Get Started with FRDR: Download a Dataset
Files for this dataset are currently being backed up so it cannot be approved at this time. Please try later.
Access to this dataset is subject to the following terms:
Creative Commons Public Domain Dedication (CC0 1.0) https://creativecommons.org/publicdomain/zero/1.0/
Citation
Lide, G., Jiajie, Z., Yao, Z., Deli, W., Juewen, L. (2023). Capture-SELEX of DNA Aptamers for Sulforhodamine B and Fluorescein. Federated Research Data Repository. https://doi.org/10.20383/103.0808
Footer Second
Footer Third
