Skip Navigation

How to Download

FRDR offers multiple ways to download datasets. Learn more in our documentation.

Capture-SELEX of DNA Aptamers for Sulforhodamine B and Fluorescein

Description: While many dye binding aptamers have been reported, most of them were for light-up aptamers
that can significantly enhance the quantum yield of fluorophores. Sulforhodamine B (SRhB) was
used as a target previously to select both DNA and RNA aptamers, and the DNA aptamer was a
G-quadruplex that can bind to a number of rhodamine analogs. In addition, the previous selections were performed by immobilizing the target molecules. In this work, we used the
library immobilization method to respectively select aptamers for SRhB and fluorescein. The
SRhB aptamer has a non-G-quadruplex structure with a Kd of 1.0 µM measured from isothermal
titration calorimetry. Upon titration of the aptamer, the fluorescence of SRhB increased 2.5-fold,
and this aptamer does not require Mg2+ for binding. Rhodamine B has even tighter binding suggesting binding through the xanthene moiety of the dyes. No binding was detected for
fluorescein. For the fluorescein selection, a dominant aptamer sequence with a Kd of 147 µM was obtained. This study provides two new aptamers for two important fluorophores that can be used to study aptamer-based separation, dye detection, and catalysis. Comparison of these aptamers also provides insights into the effect of functional groups on aptamer binding.
Authors: Lide, Gu; Xiamen University; University of Waterloo; ORCID iD 0000-0002-9466-9674
Jiajie, Zheng; University of Waterloo
Yao, Zhang; Xiamen University
Deli, Wang; Xiamen University
Juewen, Liu; University of Waterloo; ORCID iD 0000-0001-5918-9336
Keywords: Aptamers
Sulforhodamine B
Field of Research: 
Biological sciences
Analytical biochemistry
Publication Date: 2023-09-25
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Natural Sciences and Engineering Research Council of Canada (NSERC)

Files in Dataset 
No files uploaded
Download entire dataset using Globus Transfer. This method requires a Globus account and installing software. Watch Video: Get Started with FRDR: Download a Dataset
Download with Globus
Files for this dataset are currently being backed up so it cannot be approved at this time. Please try later.

Access to this dataset is subject to the following terms:
Creative Commons Public Domain Dedication (CC0 1.0)
Lide, G., Jiajie, Z., Yao, Z., Deli, W., Juewen, L. (2023). Capture-SELEX of DNA Aptamers for Sulforhodamine B and Fluorescein. Federated Research Data Repository.