Promotion of DNA Absorption onto Microplastics by Transition Metal Ions

Wu, Lyuyuan

https://ror.org/01aff2v68

University of Waterloo

Patel, Kshiti

https://ror.org/01aff2v68

University of Waterloo

Zandieh, Mohamad

https://ror.org/01aff2v68

University of Waterloo

https://orcid.org/0000-0001-9560-6184

Liu, Juewen

https://ror.org/01aff2v68

University of Waterloo

https://orcid.org/0000-0001-5918-9336

2023-03-09T11:35:31Z

2023-03-09T11:35:31Z

2023-03-09

https://www.frdr-dfdr.ca/repo/dataset/f3584ed4-2152-4c42-b207-a44f2eee20e7

https://doi.org/10.20383/102.0698

Microplastics can absorb and spread a variety of pollutants in the ecosystem posing a threat to human health. One of the common pollution sources of environmental waters is metal ions, which not only absorb on microplastics but can also promote the absorption of other invasive species such as environmental DNA. Recently, we showed that environmentally abundant metal ions (Na+, Mg2+ and Ca2+) can promote the absorption of single-stranded DNA (ssDNA) onto micro-plastics. Herein, we investigated the effect of transition metal ions including Zn2+ and Mn2+ and compared them with Mg2+ for promoting DNA adsorption. To better mimic environmental DNA, we also used a salmon sperm double-stranded DNA (dsDNA) (~2000 bp). For both ssDNA and dsDNA, the transition metals induced a higher absorption capacity compared to Mg2+, and that correlated with the higher binding affinity of transition metals to DNA. Although metal-mediated interactions were vital for ssDNA adsorption, the dsDNA absorbed on the micro-plastics even in the absence of metal ions, likely due to the abundance of binding sites of the 100-times longer dsDNA. Finally, desorption studies revealed that hydrophobic interactions were responsible for dsDNA adsorption in the absence of metal ions.

Federated Research Data Repository / dépôt fédéré de données de recherche

Creative Commons Attribution 4.0 International (CC BY 4.0)

https://creativecommons.org/licenses/by/4.0/

microplastics

water treatment

DNA

Absorption

Metal ions

Promotion of DNA Absorption onto Microplastics by Transition Metal Ions

9347409e-b290-40c8-9b35-8bc3bae5d046

/2/published/publication_693/

2023

2021-09-01/2023-02-28

Dataset

https://doi.org/10.3390/microplastics2010012

https://ror.org/01h531d29

Natural Sciences and Engineering Research Council of Canada (NSERC)

ALLRP 558435–20

Alliance Grants - Plastics Science for a Cleaner Future program

Canada First Research Excellence Fund (CFREF)

Global Water Futures Program (GWF)

RDF1040108

Chemical sciences

Analytical chemistry

Environmental chemistry (except atmospheric chemistry)

Sciences chimiques

Chimie analytique

Chimie de l'environnement (sauf la chimie de l'atmosphère)