Field
Value
Language
dc.contributor.author
Huang, Po-Jung Jimmy
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-3436-9968
en_US
dc.contributor.author
Liu, Juewen
datacite.creator.affiliationIdentifier
https://ror.org/01aff2v68
en_US
datacite.creator.affiliation
University of Waterloo
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5918-9336
en_US
dc.date.accessioned
2023-03-09T17:39:06Z
dc.date.available
2023-03-09T17:39:06Z
dc.date.issued
2023-03-09
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/fb8a55a1-7f95-419a-9fd3-bdf023e52bd6
dc.identifier.uri
https://doi.org/10.20383/102.0690
dc.description
L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need other substrates. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate and 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90% serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
DNA
en_US
dc.subject
SELEX
en_US
dc.subject
Aptamer
en_US
dc.subject
L-Lactate
en_US
dc.subject
D-Glucose
en_US
dc.subject
Biosensor
en_US
dc.title
Simultaneous Detection of L-Lactate and D-Glucose Using DNA Aptamers in Human Blood Serum
en_US
globus.shared_endpoint.name
9347409e-b290-40c8-9b35-8bc3bae5d046
globus.shared_endpoint.path
/2/published/publication_685/
datacite.publicationYear
2023
datacite.date.Collected
2021-12-01/2022-06-01
datacite.resourceType
Dataset
en_US
datacite.relatedIdentifier.IsCitedBy
https://doi.org/10.1002/anie.202212879
datacite.geolocation.geolocationPlace
University of Waterloo;Waterloo;Ontario;Canada
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada (NSERC)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
en_US
datacite.fundingReference.funderName
Canada First Research Excellence Fund (CFREF)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1060101
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Biochemistry
en_US
frdr.crdc.field_en
Analytical biochemistry
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biochimie
fr_CA
frdr.crdc.field_fr
Biochimie analytique
fr_CA
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