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The impact of fluoxetine on the gut microbiome of Pimephales promelas

Description: This data is supplementary information regarding the effects of fluoxetine on the gut microbiome of Fathead Minnows when aqueously exposed to the compound. After a 28-day exposure to three different concentrations of fluoxetine; 0.01, 10 and 100 µg/L, or control (0 µg/L), fish were euthanized, and gut contents was collected. Using DNA extraction methods, PCR amplification and sequencing, bioinformatics was then performed to interpret the metagenomic data. All tests and data were collected between September 2019 and January 2021 at the University of Saskatchewan, Saskatoon, SK, Canada. This data was generated to better understand how pharmaceuticals found downstream of wastewater treatment plants may impact the gut microbiome of aquatic organisms.
Authors: Weber, Alana; University of Saskatchewan; https://orcid.org/0000-0002-7616-4066 ORCID iD
Xie, Yuwei; University of Saskatchewan; https://orcid.org/0000-0001-5652-6413 ORCID iD
Challis, Jonathan; University of Saskatchewan
DeBofsky, Abigail; University of Saskatchewan; https://orcid.org/0000-0003-3877-6421 ORCID iD
Ankley, Phillip; University of Saskatchewan; https://orcid.org/0000-0002-5883-6148 ORCID iD
Hecker, Markus; University of Saskatchewan; https://orcid.org/0000-0002-7237-6192 ORCID iD
Jones, Paul; University of Saskatchewan
Giesy, John; University of Saskatchewan; https://orcid.org/0000-0003-1931-9962 ORCID iD
Keywords: microbiome
eDNA
pharmaceuticals
microbial community
fishes
wastewater
fluoxetine
Field of Research: 
Biological sciences
>
Microbiology
>
Microbiome

Earth and related environmental sciences
>
Natural environment sciences
>
Ecotoxicology
Date: 2-Jul-2021
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
URI: https://doi.org/10.20383/102.0412
Geographic Coverage: 
Place Name
University of Saskatchewan Aquatic Toxicology Research Facility
City
Saskatoon
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada
Geographic Point: 
Latitude
52.13375
Longitude
-106.63190
Appears in Collections:Global Water Futures

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Weber, A. , Xie, Y. , Challis, J. , DeBofsky, A. , Ankley, P. , Hecker, M. , Jones, P. , Giesy, J. (2021) The impact of fluoxetine on the gut microbiome of Pimephales promelas. Federated Research Data Repository. https://doi.org/10.20383/102.0412