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Oxidation of Fe(II) by Flavins under Anoxic Conditions

Description: This project aims to understand the redox reaction between Fe(II) and flavins. Our study demonstrated that aqueous Fe(II) can be oxidized by the oxidized form of riboflavin and flavin mononucleotide at pH 7–9. Both the rates and extents of oxidation increased with increasing concentration of oxidized flavins and increasing solution pH.

Our data are from experiments, which mainly consist of the chemical variables, were collected in the Ecohydrology lab at the University of Waterloo using the batch reaction system. All the experiments were conducted in 125 mL serum bottles at 22±1 ℃ in an anaerobic glovebox (95% N2 and 5% H2). The bottles contained Teflon-coated magnetic stir bars and were wrapped in aluminum foil to exclude light. Prior to an experiment, a solution containing 20 mM buffer and 10 mM NaCl was purged with N2 (99.998%) for at least 2 h to remove O2, then transferred to the anaerobic glovebox. Aqueous Fe2+ and uncomplexed RBF and FMN concentrations were analyzed by using a Flexstation Multimode Microplate Reader. The total concentrations of RBF and FMN were determined at 450 nm on a UV-vis spectrometer kept in the anaerobic glovebox

Additional details on the project are available in Environmental Science and Technology publication titled "Oxidation of Fe(II) by flavins under anoxic conditions" (doi: 10.1021/acs.est.0c02916)
Authors: Zhang, Peng; Water Institute; University of Waterloo; State Key Laboratory of Biogeology and Environmental Geology; China University of Geosciences; ORCID iD 0000-0003-2664-9601
Van Cappellen, Philippe; Water Institute; University of Waterloo; ORCID iD 0000-0001-5476-082
Pi, Kunfu; Water Institute; University of Waterloo; ORCID iD 0000-0002-0798-083
Keywords: Fe(II)
flavins
oxidation
alkaline
pH
Field of Research: 
Earth and related environmental sciences
>
Geochemistry
>
Environmental geochemistry
Publication Date: 2021-02-16
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Canada Excellence Research Chairs (CERC); Canada Excellence Research Chair in Ecohydrology
Natural Sciences and Engineering Research Council of Canada (NSERC); STPGP-494652-16
National Natural Science Foundation of China
URI: https://doi.org/10.20383/102.0320
Related Identifiers: 
This dataset is a supplement to
Appears in Collections:Water Institute

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Zhang, P., Van Cappellen, P., Pi, K. (2021). Oxidation of Fe(II) by Flavins under Anoxic Conditions. Federated Research Data Repository. https://doi.org/10.20383/102.0320