How to Download
FRDR offers multiple ways to download datasets. Learn more in our documentation.
DNA aptamers for common buffer molecules: possibility of buffer interference in SELEX
Description: | During a typical aptamer selection experiment, buffer molecules are used at 10 to 50 mM, whereas target molecules could be used at much lower concentrations even in low µM levels. Therefore, doubts existed regarding the potential enrichment of buffer binding aptamers, particularly for failed selections that cannot validate binding of enriched sequences. In this study, we respectively used two common buffer molecules, Tris and HEPES, as target molecules. While we successfully isolated aptamers for Tris buffer, our attempts to generate aptamers for HEPES buffer failed. Thioflavin T (ThT) fluorescence spectroscopy confirmed the dissociation constant of the Tris buffer aptamer to be 2.9 mM, while isothermal titration calorimitry showed a Kd of 43 µM. NMR spectroscopy also confirmed aptamer binding. Finally, we discussed the implications of this buffer selection work and recommended the use of certain buffers. |
Authors: | Ding, Yuzhe; University of Waterloo Zhang, Ziyu; University of Waterloo Kaiyum, Yunus; York University; ![]() Heng, Yicheng; University of Waterloo Johnson, Philip; York University; ![]() Liu, Juewen; University of Waterloo; ![]() |
Keywords: | DNA SELEX Aptamer Tris HEPES |
Field of Research: | Biological sciences > Biochemistry > Analytical biochemistry
|
Publication Date: | 2025-01-15 |
Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
URI: | https://doi.org/10.20383/103.01054 |
Related Identifiers: |
This dataset is cited by
|
Appears in Collections: | Water Institute |
Files in Dataset
No files uploaded
Download entire dataset using Globus Transfer. This method requires a Globus account and installing software. Watch Video: Get Started with FRDR: Download a Dataset
Files for this dataset are currently being backed up so it cannot be approved at this time. Please try later.
Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Ding, Y., Zhang, Z., Kaiyum, Y., Heng, Y., Johnson, P., Liu, J. (2025). DNA aptamers for common buffer molecules: possibility of buffer interference in SELEX. Federated Research Data Repository. https://doi.org/10.20383/103.01054