Field
Value
Language
dc.contributor.author
Su, Geoffrey, Y.
datacite.creator.affiliationIdentifier
https://ror.org/0213rcc28
en_US
datacite.creator.affiliation
Simon Fraser University
en_US
datacite.creator.nameIdentifier
https://orcid.org/0009-0005-8863-0086
en_US
dc.contributor.author
Allison, Michael, J.
datacite.creator.affiliationIdentifier
https://ror.org/04s5mat29
en_US
datacite.creator.affiliation
University of Victoria
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Beblow, Jordan
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Gitanyow Fisheries Authority
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Koch, Kevin
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Gitanyow Fisheries Authority
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Anderson, Jeffrey
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Geomorphic Environmental Services
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
M’Gonigle, Leithen, K.
datacite.creator.affiliationIdentifier
https://ror.org/0213rcc28
en_US
datacite.creator.affiliation
Simon Fraser University
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Cleveland, Mark
datacite.creator.affiliationIdentifier
en_US
datacite.creator.affiliation
Gitanyow Fisheries Authority
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Helbing, Caren, C.
datacite.creator.affiliationIdentifier
https://ror.org/04s5mat29
en_US
datacite.creator.affiliation
University of Victoria
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Marlatt, Vicki, L.
datacite.creator.affiliationIdentifier
https://ror.org/0213rcc28
en_US
datacite.creator.affiliation
Simon Fraser University
en_US
datacite.creator.nameIdentifier
en_US
dc.coverage.temporal
2021-06-21/2021-11-07
dc.date.accessioned
2023-05-11T17:56:33Z
dc.date.available
2023-05-11T17:56:33Z
dc.date.issued
2023-05-11
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/1dc2ed5c-f679-4162-8486-ef7acd976c45
dc.identifier.uri
https://doi.org/10.20383/102.0727
dc.description
The field of environmental DNA has advanced over the past decade, with multiple approaches available for a variety of sampling media and species. Here, we quantify salmon environmental DNA upstream of a fish counting fence along with river velocity, and together, use these values to determine the correlation between the number of salmon passing by the fish fence daily with daily eDNA rates in water before, during and after the salmon spawning season for 4 Pacific salmonids (Oncorhynchus gorbuscha, O. kisutch, O. tshawytscha, O. nerka; pink, coho, Chinook, sockeye respectively).
Hydrogeological data of streamflow and water temperature were measured in 10 minutes intervals by automatic barologgers and levelloggers and averaged over a 24-hour period between 9pm the previous day and 9pm to get daily temperature and streamflow rate.
Triplicate daily water samples were 50 m upstream of the fish counting fence in bleach sterilised glass bottles and filtered on 0.45 micron cellulose filters before storing in envelopes placed in desiccant. DNA is then extracted from these filters with stringent contamination protocols using DNeasy Blood & Tissue kits. qPCR is performed using primers and probes for each of the 4 salmonid species and the Cq values generated are converted to copies/L (S ̂, sHat) using either linear regression using parameters derived from n=24 replicate tests or by publicly available eLowQuant method (https://github.com/mlespera/eLowQuant) depending on the number of non-zero reads out of each 8 technical replicate test. No amplification producing no Cq value is an absence of target DNA.
The copies/L readings for each daily triplicate water sample are then averaged and multiplied by the daily stream flow rate to get flow adjusted eDNA rate. This can then by log transformed by doing log_10〖(S ̂ 〗+1) as to not lose readings of 0 copies/L.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Public Domain Dedication (CC0 1.0)
en_US
dc.rights.uri
https://creativecommons.org/publicdomain/zero/1.0/
en_US
dc.subject
eDNA
en_US
dc.subject
environmental DNA
en_US
dc.subject
salmon
en_US
dc.subject
enumeration
en_US
dc.title
Enumeration Potential of Environmental DNA for Pacific Salmon Stock Assessments using qPCR based methodologies converting Cq into copy number using linear regression and the eLowQuant method
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/8/published/publication_722/
datacite.publicationyear
2023
datacite.contributor.DataCollector
Michael J. Allison
datacite.contributor.DataCollector
Jordan Beblow
datacite.contributor.DataCollector
Kevin Koch
datacite.contributor.DataCollector
Jeffrey Anderson
datacite.contributor.ProjectManager
Mark Cleveland
datacite.contributor.ProjectManager
Caren C. Helbing
datacite.contributor.ProjectManager
Vicki L. Marlatt
datacite.contributor.Other
Leithen K. M'Gonigle
datacite.date.Collected
2021-06-21/2021-11-07
datacite.resourcetype
Dataset
en_US
datacite.relatedidentifier.IsCitedBy
https://doi.org/10.1002/edn3.508
datacite.geolocation.geolocationPoint
55.1173725 -128.0483541
datacite.geolocation.geolocationPlace
Kitwanga River;Kitwanga;British Columbia;Canada
datacite.fundingReference.funderIdentifier
https://ror.org/03gne5057
en_US
datacite.fundingReference.funderName
Genome British Columbia
en_US
datacite.fundingReference.awardNumber
SIP024
en_US
datacite.fundingReference.awardTitle
Sector Innovation Program
en_US
frdr.crdc.code
RDF4010502
en_US
frdr.crdc.group_en
Agriculture, forestry, and fisheries
en_US
frdr.crdc.class_en
Fisheries sciences
en_US
frdr.crdc.field_en
Aquatic ecosystem studies and stock assessment
en_US
frdr.crdc.group_fr
Agriculture, foresterie et pêches
fr_CA
frdr.crdc.class_fr
Sciences de la pêche
fr_CA
frdr.crdc.field_fr
Études des écosystèmes aquatiques et estimation des stocks
fr_CA
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