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Wastewater discharges alter microbial community composition in surface waters of the Canadian prairies

Description: Freshwater ecosystems occupy only a small portion of the Earth's surface, but harbor a disproportionate amount of biodiversity that is particularly threatened by wastewater discharges, as one of the most common anthropogenic impact on these systems. As wastewater effluents are also sources of antimicrobial agents and other microorganisms, they reflect a particular threat to natural microbial communities within receiving rivers. Our knowledge about the impact of wastewater effluents on these communities is, however, largely unexplored. In this study, composition of microbial communities upstream and downstream of 5 different wastewater treatment plants within Southern Saskatchewan, Canada were examined. Three matrices, the water column, sediments, and biofilms attached to hard surfaces, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata.
Authors: Esser, Milena; University of Saskatchewan; https://orcid.org/0000-0002-2802-6160 ORCID iD
Hoggarth, Cameron; Global Institute for Water Security; University of Saskatchewan; https://orcid.org/0000-0002-1275-5334 ORCID iD
Baulch, Helen; Global Institute for Water Security; University of Saskatchewan; https://orcid.org/0000-0001-9018-4998 ORCID iD
Challis, Jonathan K.; University of Saskatchewan; https://orcid.org/0000-0003-3514-0647 ORCID iD
Xie, Yuwei; University of Saskatchewan; https://orcid.org/0000-0001-5652-6413 ORCID iD
Giesy, John P.; University of Saskatchewan; Baylor University; Michigan State University; https://orcid.org/0000-0003-1931-9962 ORCID iD
Hecker, Markus; University of Saskatchewan; https://orcid.org/0000-0002-7237-6192 ORCID iD
Brinkmann, Markus; Global Institute for Water Security; University of Saskatchewan; https://orcid.org/0000-0002-4985-263X ORCID iD
Keywords: Microbial community analysis
Metabarcoding
Wastewater
Pharmaceuticals
Field of Research: 
Environmental biotechnology
>
Environmental biotechnology
>
Environmental biotechnology diagnostics (including biosensors)
Date: 19-Aug-2022
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
URI: https://doi.org/10.20383/103.0616
Geographic Coverage: 
Place Name
South Saskatchewan River, upstream WWTP
City
Saskatoon
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
South Saskatchewan River, downstream WWTP
City
Saskatoon
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
Wascana Creek, upstream WWTP
City
Regina
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
Wascana Creek, downstream WWTP
City
Regina
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
Moose Jaw River, upstream WWTP
City
Moose Jaw
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
Moose Jaw River, downstream WWTP
City
Moose Jaw
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
North Saskatchewan River, upstream WWTP
City
Prince Albert
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
North Saskatchewan River, downstream WWTP
City
Prince Albert
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
Swift Current Creek, upstream WWTP
City
Swift Current
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada

Place Name
Swift Current Creek, downstream WWTP
City
Swift Current
Province /
Province / Territory
Saskatchewan
Territory
 
Country
Canada
Geographic Point: 
Latitude
52.3185753
Longitude
-106.4611579

Latitude
51.9838014
Longitude
-106.7362366

Latitude
50.476712
Longitude
-104.732583

Latitude
50.481960
Longitude
-104.778461

Latitude
50.303740
Longitude
-105.449218

Latitude
50.397031
Longitude
-105.452965

Latitude
53.210545
Longitude
-105.704689

Latitude
53.235300
Longitude
-105.550524

Latitude
50.2822670
Longitude
-107.7786335

Latitude
50.345973
Longitude
-107.701144
Appears in Collections:Global Water Futures

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Esser, M. , Hoggarth, C. , Baulch, H. , Challis, J. , Xie, Y. , Giesy, J. , Hecker, M. , Brinkmann, M. (2022) Wastewater discharges alter microbial community composition in surface waters of the Canadian prairies. Federated Research Data Repository. https://doi.org/10.20383/103.0616