Wastewater discharges alter microbial community composition in surface waters of the Canadian prairies

Esser, Milena

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0002-2802-6160

Hoggarth, Cameron

https://ror.org/05997db74;https://ror.org/010x8gc63

Global Institute for Water Security;University of Saskatchewan

https://orcid.org/0000-0002-1275-5334

Baulch, Helen

https://ror.org/05997db74;https://ror.org/010x8gc63

Global Institute for Water Security;University of Saskatchewan

https://orcid.org/0000-0001-9018-4998

Challis, Jonathan K.

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0003-3514-0647

Xie, Yuwei

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0001-5652-6413

Giesy, John P.

https://ror.org/010x8gc63;https://ror.org/005781934;https://ror.org/05hs6h993

University of Saskatchewan;Baylor University;Michigan State University

https://orcid.org/0000-0003-1931-9962

Hecker, Markus

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0002-7237-6192

Brinkmann, Markus

https://ror.org/05997db74;https://ror.org/010x8gc63

Global Institute for Water Security;University of Saskatchewan

https://orcid.org/0000-0002-4985-263X

2022-08-19T15:35:57Z

2022-08-19T15:35:57Z

2022-08-19

https://www.frdr-dfdr.ca/repo/dataset/4a0ea2d4-10c2-4d50-9f4c-91d286d0ca02

https://doi.org/10.20383/103.0616

Freshwater ecosystems occupy only a small portion of the Earth's surface, but harbor a disproportionate amount of biodiversity that is particularly threatened by wastewater discharges, as one of the most common anthropogenic impact on these systems. As wastewater effluents are also sources of antimicrobial agents and other microorganisms, they reflect a particular threat to natural microbial communities within receiving rivers. Our knowledge about the impact of wastewater effluents on these communities is, however, largely unexplored. In this study, composition of microbial communities upstream and downstream of 5 different wastewater treatment plants within Southern Saskatchewan, Canada were examined. Three matrices, the water column, sediments, and biofilms attached to hard surfaces, were analyzed. The samples were extracted for DNA and were PCR amplified targeting the hypervariable V3-V4 region of the 16S ribosomal RNA subunit I gene of prokaryotes, as well as the hypervariable V3 region of the 18S ribosomal RNA gene of eukaryotic organisms. Amplicons were sequenced performing a 600-cycle paired-end sequencing run on an Illumina® MiSeq sequencer. This dataset includes the demultiplexed sequencing output, the feature table with taxonomic annotation, and the sample metadata.

Federated Research Data Repository / dépôt fédéré de données de recherche

Creative Commons Attribution 4.0 International (CC BY 4.0)

https://creativecommons.org/licenses/by/4.0/

Microbial community analysis

Metabarcoding

Wastewater

Pharmaceuticals

Wastewater discharges alter microbial community composition in surface waters of the Canadian prairies

515c70c4-2eb8-4f2a-b406-7959b5edc28d

/6/published/publication_611/

2022

2021-06-16/2021-07-12

Dataset

52.3185753 -106.4611579

51.9838014 -106.7362366

50.476712 -104.732583

50.481960 -104.778461

50.303740 -105.449218

50.397031 -105.452965

53.210545 -105.704689

53.235300 -105.550524

50.2822670 -107.7786335

50.345973 -107.701144

South Saskatchewan River, upstream WWTP;Saskatoon;Saskatchewan;Canada

South Saskatchewan River, downstream WWTP;Saskatoon;Saskatchewan;Canada

Wascana Creek, upstream WWTP;Regina;Saskatchewan;Canada

Wascana Creek, downstream WWTP;Regina;Saskatchewan;Canada

Moose Jaw River, upstream WWTP;Moose Jaw;Saskatchewan;Canada

Moose Jaw River, downstream WWTP;Moose Jaw;Saskatchewan;Canada

North Saskatchewan River, upstream WWTP;Prince Albert;Saskatchewan;Canada

North Saskatchewan River, downstream WWTP;Prince Albert;Saskatchewan;Canada

Swift Current Creek, upstream WWTP;Swift Current;Saskatchewan;Canada

Swift Current Creek, downstream WWTP;Swift Current;Saskatchewan;Canada

https://ror.org/01gavpb45

Canadian Institutes of Health Research (CIHR)

419205

Global Water Futures Program (GWF)

RDF2090104

Environmental biotechnology

Environmental biotechnology

Environmental biotechnology diagnostics (including biosensors)

Biotechnologies de l'environnement

Biotechnologies de l'environnement

Outils de diagnostic en biotechnologies de l'environnement (y compris les biocapteurs)