How to Download
FRDR offers multiple ways to download datasets. Learn more in our documentation.
Selection of Aptamers for Sensing Caffeine and Discrimination of Its Three Single Demethylated Analogues
| Description: | With the growing consumption of caffeine-containing beverages, detection of caffeine has become an important biomedical, bioanalytical, and environmental topic. Four high-quality aptamers for caffeine were isolated and were carefully characterized. A structure-switching fluorescent sensor was designed showing a detection limit of 1.2 μM caffeine, which reflected the labeled caffeine concentration within 6.1% difference for eight commercial beverages. In 20% human serum, a detection limit of 4.0 μM caffeine was achieved. In addition, these four fluorescent aptamer sensors can form a sensing array, caffeine and the three analogues were well separated from nine other closely related molecules. |
| Notes: | Dataset is from the publication "Selection of Aptamers for Sensing Caffeine and Discrimination of Its Three Single Demethylated Analogues" https://doi.org/10.1021/acs.analchem.1c04349 Item exited embargo and became publicly available on 2022-06-10 |
| Authors: | Huang, Po-Jung Jimmy; University of Waterloo;  0000-0003-3436-9968Liu, Juewen; University of Waterloo; 0000-0001-5918-9336 |
| Keywords: | DNA SELEX fluorescence Aptamer Caffeine Biosensor |
| Field of Research: | Biological sciences > Biochemistry > Analytical biochemistry
|
| Publication Date: | 2022-06-10 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council of Canada; Canada First Research Excellence Fund |
| URI: | https://doi.org/10.20383/103.0587 |
| Related Identifiers: |
This dataset is cited by
|
| Geographic Coverage: | Place Name University of Waterloo City Waterloo Province / Province / Territory Ontario Territory Country Canada |
| Appears in Collections: | Water Institute |
Files in Dataset
No files uploaded
Download entire dataset using Globus Transfer. This method requires a Globus account and installing software. Watch Video: Get Started with FRDR: Download a Dataset
Files for this dataset are currently being backed up so it cannot be approved at this time. Please try later.
Access to this dataset is subject to the following terms:
Creative Commons Public Domain Dedication (CC0 1.0) https://creativecommons.org/publicdomain/zero/1.0/
Citation
Huang, P., Liu, J. (2022). Selection of Aptamers for Sensing Caffeine and Discrimination of Its Three Single Demethylated Analogues. Federated Research Data Repository. https://doi.org/10.20383/103.0587
Footer Second
Footer Third
