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Metal-Mediated DNA Adsorption on Carboxylated, Hydroxylated and Hydrogenated Nanodiamonds
| Description: | Nanodiamonds (NDs) have attracted considerable attention owing to their quantum properties and versatility in biological applications. In this study, we systematically investigated the adsorption of DNA oligonucleotides onto NDs with three types of surface groups: carboxylated (COOH-), hydroxylated (OH-), and hydrogenated (H-). Among them, only the H-NDs showed fluorescence quenching property that is useful for real-time DNA adsorption kinetic studies. The effect of common metal ions on DNA adsorption was studied. In the presence of Na+, the order of DNA adsorption efficiency was H-> OH- > COOH-, whereas all the NDs showed a similar DNA adsorption efficiency in the presence of divalent metal ions such as Ca2+ and Zn2+. Desorption studies revealed that hydrogen bonding and metal-mediated interactions were dominant for the adsorption of DNA, and the H-NDs exhibited extraordinarily tight DNA adsorption. Finally, a fluorescently labeled DNA was adsorbed on NDs for DNA detection, and the COOH-NDs had the highest target specificity, and a detection limit of 1.4 nM was achieved. This study indicates the feasibility of using metal ions to mediate the physical adsorption of DNA to NDs and compares various NDs with graphene oxide for fundamental understanding. |
| Authors: | Zandieh, Mohamad; University of Waterloo;  0000-0001-9560-6184Liu, Juewen; University of Waterloo; 0000-0001-5918-9336 |
| Keywords: | Nanodiamonds DNA Oligonucleotides Adsorption Surface Science |
| Field of Research: | Chemical sciences > Analytical chemistry > Sensor technology (chemical aspects)
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| Publication Date: | 2023-08-17 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council of Canada Global Water Futures |
| URI: | https://doi.org/10.20383/103.0773 |
| Related Identifiers: |
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Citation
Zandieh, M., Liu, J. (2023). Metal-Mediated DNA Adsorption on Carboxylated, Hydroxylated and Hydrogenated Nanodiamonds. Federated Research Data Repository. https://doi.org/10.20383/103.0773
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