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Metal-Mediated DNA Adsorption on Carboxylated, Hydroxylated and Hydrogenated Nanodiamonds

Description: Nanodiamonds (NDs) have attracted considerable attention owing to their quantum properties and versatility in biological applications. In this study, we systematically investigated the adsorption of DNA oligonucleotides onto NDs with three types of surface groups: carboxylated (COOH-), hydroxylated (OH-), and hydrogenated (H-). Among them, only the H-NDs showed fluorescence quenching property that is useful for real-time DNA adsorption kinetic studies. The effect of common metal ions on DNA adsorption was studied. In the presence of Na+, the order of DNA adsorption efficiency was H-> OH- > COOH-, whereas all the NDs showed a similar DNA adsorption efficiency in the presence of divalent metal ions such as Ca2+ and Zn2+. Desorption studies revealed that hydrogen bonding and metal-mediated interactions were dominant for the adsorption of DNA, and the H-NDs exhibited extraordinarily tight DNA adsorption. Finally, a fluorescently labeled DNA was adsorbed on NDs for DNA detection, and the COOH-NDs had the highest target specificity, and a detection limit of 1.4 nM was achieved. This study indicates the feasibility of using metal ions to mediate the physical adsorption of DNA to NDs and compares various NDs with graphene oxide for fundamental understanding.
Authors: Zandieh, Mohamad; University of Waterloo; ORCID iD 0000-0001-9560-6184
Liu, Juewen; University of Waterloo; ORCID iD 0000-0001-5918-9336
Keywords: Nanodiamonds
Surface Science
Field of Research: 
Chemical sciences
Analytical chemistry
Sensor technology (chemical aspects)
Publication Date: 2023-08-17
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Natural Sciences and Engineering Research Council
Global Water Futures
Related Identifiers: 

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Zandieh, M., Liu, J. (2023). Metal-Mediated DNA Adsorption on Carboxylated, Hydroxylated and Hydrogenated Nanodiamonds. Federated Research Data Repository.