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Genotype and raw sequencing data for "Development of a high-density sub-species-specific targeted SNP assay for Rocky Mountain bighorn sheep (Ovis canadensis canadensis)"

Description: We designed a novel SNP (Single nucleotide polymorphism) genotyping assay for use with Tecan's Allegro genotyping technology (a form of single primer enrichment technology or SPET) for Rocky Mountain bighorn sheep. Our overall goal was to develop an assay which targeted 50,000 SNPs in this species but during development we designed a trial 10,000 SNP assay. We trialled both the 10,000 and 50,000 SNP assay on 40 Rocky Mountain bighorn sheep, eight individuals from two other subspecies of bighorn sheep, and eight individuals from two other species of thinhorn sheep. The Tecan library prep was performed on these samples and then sequenced on an Illumina NextSeq next generation sequencer. Data here contains raw sequencing reads for the 56 individuals genotyped using the 10,000 SNP and 50,000 SNP assays, and the VCF genotype files produced when aligning and SNP calling was performed on these reads.
Authors: Deakin, Samuel; University of Alberta; University of Calgary; ORCID iD 0000-0003-4227-8767
Coltman, David; University of Alberta; Western University; ORCID iD 0000-0002-1268-2307
Keywords: genomics
sequence data
genetics
bighorn
sheep
tecan
allegro
SNP
Single Nucleotide Polymorphism (SNP)
Field of Research: 
Biological sciences
>
Genetics
>
Conservation genetics
Publication Date: 2024-02-07
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Natural Sciences and Engineering Research Council of Canada
University of Alberta
Canadian Mountain Network
URI: https://doi.org/10.20383/103.0859
Geographic Coverage: 
Place Name
Alberta
Province /
Province / Territory
Alberta
Territory
 
Country
Canada

Place Name
Wyoming
State
Wyoming
Country
United States

Place Name
Montana
State
Montana
Country
United States

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Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Deakin, S., Coltman, D. (2024). Genotype and raw sequencing data for "Development of a high-density sub-species-specific targeted SNP assay for Rocky Mountain bighorn sheep (Ovis canadensis canadensis)". Federated Research Data Repository. https://doi.org/10.20383/103.0859