Field
Value
Language
dc.contributor.author
James, Benson
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-1819-1899
en_US
dc.contributor.author
Iqra, Azam
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
en_US
dc.date.accessioned
2023-09-13T20:39:19Z
dc.date.available
2023-09-18
dc.date.issued
2023-09-18
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/a34d99ad-9ee6-48d8-97a5-cb383f9ed501
dc.identifier.uri
https://doi.org/10.20383/103.0800
dc.description
Data for submitted manuscript. Abstract: Background. Monitoring cellular processes across different levels of complexity, from the cellular to the tissue scale, is important for understanding tissue structure and function. However, it is challenging to monitor and estimate these structural and dynamic interactions within three-dimensional (3D) tissue models.
Objective. The aim of this study was to design a method for imaging, tracking, and quantifying 3D changes in cell morphology (shape and size) within liver tissue, specifically a precision-cut liver slice (PCLS). A PCLS is a three-dimensional (3D) model of the liver that allows the study of the structure and function of liver cells in their native microenvironment.
Methods. Here we present a method for imaging liver tissue during anisosmotic exposure in a multispectral four-dimensional manner. Three metrices of tissue morphology were measured to quantify the effects of osmotic stress on liver tissue. We estimated the changes in the volume of whole precision cut liver slices (PCLS), quantified the changes in nuclei position, and calculated the changes in volumetric responses of tissue-embedded cells.
Results. During equilibration with cell-membrane-permeating and non-permeating solutes, the whole tissue experiences shrinkage and expansion. As nuclei showed a change in position and directional displacement under osmotic stress, we demonstrate that nuclei could be used as a probe to measure local osmotic and mechanical stress. Moreover, we demonstrate that cells change their volume within tissue slices as a result of osmotic perturbation and that this change in volume is dependent on the position of the cell within the tissue and the duration of the exposure. Conclusion. The results of this study have implications for a better understanding of multiscale transport, mechanobiology, and triggered biological responses within complex biological structures.
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by-nc/4.0/
en_US
dc.subject
4D Confocal
en_US
dc.subject
Cryoprotectant
en_US
dc.subject
Mass Transport
en_US
dc.subject
Nuclei volume
en_US
dc.title
Multiscale transport and 4D time-lapse imaging in Precision Cut Liver Slices (PCLS)-Supplementary Data
en_US
globus.shared_endpoint.name
f163c1b3-9c88-42f6-a7bb-5839ed6c4063
globus.shared_endpoint.path
/1/published/publication_795/
datacite.publicationyear
2023
datacite.contributor.Supervisor
James Benson
datacite.date.Collected
2022-02-01/2022-12-15
datacite.resourcetype
Dataset
en_US
datacite.geolocation.geolocationPlace
University of Saskatchewan;Saskatoon;Saskatchewan;Canada
datacite.fundingReference.funderIdentifier
https://ror.org/01h531d29
en_US
datacite.fundingReference.funderName
Natural Sciences and Engineering Research Council of Canada
en_US
datacite.fundingReference.awardNumber
RGPIN-2023-04007
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderIdentifier
https://ror.org/01gavpb45
en_US
datacite.fundingReference.funderName
Canadian Institutes of Health Research
en_US
datacite.fundingReference.awardNumber
PJT - 175283
en_US
datacite.fundingReference.awardTitle
en_US
frdr.crdc.code
RDF1060203
en_US
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Cell biology
en_US
frdr.crdc.field_en
Cellular interactions (including adhesion, matrix and cell wall)
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Biologie cellulaire
fr_CA
frdr.crdc.field_fr
Interactions cellulaires (y compris l'adhérence, la matrice et la paroi cellulaire)
fr_CA
datacite.description.other
Item exited embargo and became publicly available on 2023-09-18