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Multiscale transport and 4D time-lapse imaging in Precision Cut Liver Slices (PCLS)-Supplementary Data
| Description: | Data for submitted manuscript. Abstract: Background. Monitoring cellular processes across different levels of complexity, from the cellular to the tissue scale, is important for understanding tissue structure and function. However, it is challenging to monitor and estimate these structural and dynamic interactions within three-dimensional (3D) tissue models. Objective. The aim of this study was to design a method for imaging, tracking, and quantifying 3D changes in cell morphology (shape and size) within liver tissue, specifically a precision-cut liver slice (PCLS). A PCLS is a three-dimensional (3D) model of the liver that allows the study of the structure and function of liver cells in their native microenvironment. Methods. Here we present a method for imaging liver tissue during anisosmotic exposure in a multispectral four-dimensional manner. Three metrices of tissue morphology were measured to quantify the effects of osmotic stress on liver tissue. We estimated the changes in the volume of whole precision cut liver slices (PCLS), quantified the changes in nuclei position, and calculated the changes in volumetric responses of tissue-embedded cells. Results. During equilibration with cell-membrane-permeating and non-permeating solutes, the whole tissue experiences shrinkage and expansion. As nuclei showed a change in position and directional displacement under osmotic stress, we demonstrate that nuclei could be used as a probe to measure local osmotic and mechanical stress. Moreover, we demonstrate that cells change their volume within tissue slices as a result of osmotic perturbation and that this change in volume is dependent on the position of the cell within the tissue and the duration of the exposure. Conclusion. The results of this study have implications for a better understanding of multiscale transport, mechanobiology, and triggered biological responses within complex biological structures. |
| Notes: | Item exited embargo and became publicly available on 2023-09-18 |
| Authors: | James, Benson; University of Saskatchewan;  0000-0003-1819-1899Iqra, Azam; University of Saskatchewan |
| Keywords: | 4D Confocal Cryoprotectant Mass Transport Nuclei volume |
| Field of Research: | Biological sciences > Cell biology > Cellular interactions (including adhesion, matrix and cell wall)
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| Publication Date: | 2023-09-18 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council; RGPIN-2023-04007 Canadian Institutes of Health Research; PJT - 175283 |
| URI: | https://doi.org/10.20383/103.0800 |
| Geographic Coverage: | Place Name University of Saskatchewan City Saskatoon Province / Province / Territory Saskatchewan Territory Country Canada |
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Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/
Citation
James, B., Iqra, A. (2023). Multiscale transport and 4D time-lapse imaging in Precision Cut Liver Slices (PCLS)-Supplementary Data. Federated Research Data Repository. https://doi.org/10.20383/103.0800
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