How to Download
FRDR offers multiple ways to download datasets. Learn more in our documentation.
4D confocal microscopy of time dependent cell and nuclear osmotic response reveals differential osmoregulation and transport kinetics
| Description: | We investigated the equilibrium and dynamic cell and nuclear volumetric responses in adherent human hepatoblastoma (HepG2) cell monolayers using live three-dimensional imaging. We have further demonstrated that both cells and nuclei regulate their volume in response to osmotic stress. For instance, during hyposmotic stress, cells swell and lose important osmolytes through active volume regulation or bleb formation, however, nuclei undergo more expansion than cells. Whereas during hyperosmotic stress, both cells and nuclei shrink rapidly into irregular shapes and undergo passive volume regulation. Furthermore, we explored the mechanism of osmoregulation in these cells by restoring isosmotic conditions after osmotic stress and found a higher return-to-equilibrium volume for nuclei compared to their initial isosmotic volume, while cytosol returned to a lower volume than its isosmotic volume after swelling. Moreover, water and solute permeability estimates for both cells and nuclei were also inferred from exposure to different anisosmotic media by fitting coupled transport models. This accurate estimation of cell and nuclear volumetric responses and water and solute permeability parameters could have important implications for understanding cell physiology and the mechanism of osmoregulation. |
| Authors: | Azam, Iqra; University of Saskatchewan Olver, Dominic; University of Saskatchewan Benson, James; University of Saskatchewan;  0000-0003-1819-1899 |
| Keywords: | Cell volume Osmotic stress Three-dimensional imaging Image analysis Nuclear biophysical parameters |
| Field of Research: | Biological sciences > Cell biology > Cell biology, not elsewhere classified
|
| Publication Date: | 2023-11-15 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| URI: | https://doi.org/10.20383/103.0843 |
| Geographic Coverage: | Place Name University of Saskatchewan City Saskatoon Province / Province / Territory Saskatchewan Territory Country Canada |
| Geographic Point: | Latitude 52.132854 Longitude -106.631401 |
Files in Dataset
No files uploaded
Download entire dataset using Globus Transfer. This method requires a Globus account and installing software. Watch Video: Get Started with FRDR: Download a Dataset
Files for this dataset are currently being backed up so it cannot be approved at this time. Please try later.
Access to this dataset is subject to the following terms:
Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/
Citation
Azam, I., Olver, D., Benson, J. (2023). 4D confocal microscopy of time dependent cell and nuclear osmotic response reveals differential osmoregulation and transport kinetics. Federated Research Data Repository. https://doi.org/10.20383/103.0843
Footer Second
Footer Third
