Skip Navigation

RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures

Description: Zooplankton metabarcoding can provide a high-throughput method for characterizing community response to environmental stressors. There are issues, however, with inferring actual response to stressors using DNA metabarcoding as it cannot distinguish alive organisms within bulk samples. Here we used normalized vitality, namely RNA metabarcoding normalized by DNA metabarcoding, to characterize the zooplankton community response to environmental influence. DNA and RNA metabarcoding was also applied in the context of assessing response of the zooplankton community exposed to simulated spills of diluted bitumen (dilbit), with experimental remediation practices of enhanced monitored natural recovery and shoreline cleaner application. Zooplankton samples were collected via pump on days -3 and 11 and 38 days after the simulated dilbit spill. The zooplankton samples were co-extracted for DNA and RNA and were PCR amplified targeting the mitochondrial Cytochrome c Oxidase subunit I gene (CO1) region, with amplicon sequencing following. The dataset includes the demultiplexed sequencing output, the feature table with species-level taxonomic annotation, and the sample metadata. This dataset contains data from enclosures in rock and cobble substrate. Note that a similar study was conducted for wetland habitat enclosures, with different analyses and interpretation being conducted on the data (dataset available at https://doi.org/10.20383/101.0313).
Notes: Item exited embargo and became publicly available on 2021-12-01
L’élément est sorti de sa période d’embargo et est devenu public le 2021-12-01
Authors: Ankley, Phillip; University of Saskatchewan; https://orcid.org/0000-0002-5883-6148 ORCID iD
Xie, Yuwei; University of Saskatchewan; https://orcid.org/0000-0001-5652-6413 ORCID iD
Havens, Sonya; International Institute for Sustainable Development
Peters, Lisa; University of Manitoba; https://orcid.org/0000-0002-4310-3136 ORCID iD
Timlick, Lauren; International Institute for Sustainable Development
Rodriguez-Gil, Jose Luis; International Institute for Sustainable Development; https://orcid.org/0000-0001-9325-9131 ORCID iD
Giesy, John; University of Saskatchewan; https://orcid.org/0000-0003-1931-9962 ORCID iD
Palace, Vince; International Institute for Sustainable Development; https://orcid.org/0000-0003-2134-2299 ORCID iD
Keywords: metabarcoding
zooplankton
ecogenomics
Cytochrome c Oxidase subunit 1
diluted bitumen
remediation
boreal
freshwater
community RNA
Date: 1-Nov-2021
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
URI: https://doi.org/10.20383/102.0332
Related Identifiers: 
Geographic Coverage: 
Place Name
ELA Lake 260
Province /
Province / Territory
Ontario
Territory
 
Country
Canada
Geographic Point: 
Latitude
49.6964
Longitude
-93.76752
Appears in Collections:Global Water Futures

Files in Dataset 
No files uploaded

Files for this dataset are currently being backed up so it cannot be approved at this time. Please try later.
Access to this dataset is subject to the following terms:
Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Citation
Ankley, P. , Xie, Y. , Havens, S. , Peters, L. , Timlick, L. , Rodriguez-Gil, J. , Giesy, J. , Palace, V. (2021) RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures. Federated Research Data Repository. https://doi.org/10.20383/102.0332