RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures

Ankley, Phillip

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0002-5883-6148

Xie, Yuwei

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0001-5652-6413

Havens, Sonya

https://ror.org/043zcks33

International Institute for Sustainable Development

Peters, Lisa

https://ror.org/02gfys938

University of Manitoba

https://orcid.org/0000-0002-4310-3136

Timlick, Lauren

https://ror.org/043zcks33

International Institute for Sustainable Development

Rodriguez-Gil, Jose Luis

https://ror.org/043zcks33

International Institute for Sustainable Development

https://orcid.org/0000-0001-9325-9131

Giesy, John

https://ror.org/010x8gc63

University of Saskatchewan

https://orcid.org/0000-0003-1931-9962

Palace, Vince

https://ror.org/043zcks33

International Institute for Sustainable Development

https://orcid.org/0000-0003-2134-2299

2021-03-18T19:37:07Z

2021-11-01T06:00:00Z

2021-11-01

https://www.frdr-dfdr.ca/repo/dataset/ea927258-85a7-451a-8ed8-fff82a690b7b

https://doi.org/10.20383/102.0332

Zooplankton metabarcoding can provide a high-throughput method for characterizing community response to environmental stressors. There are issues, however, with inferring actual response to stressors using DNA metabarcoding as it cannot distinguish alive organisms within bulk samples. Here we used normalized vitality, namely RNA metabarcoding normalized by DNA metabarcoding, to characterize the zooplankton community response to environmental influence. DNA and RNA metabarcoding was also applied in the context of assessing response of the zooplankton community exposed to simulated spills of diluted bitumen (dilbit), with experimental remediation practices of enhanced monitored natural recovery and shoreline cleaner application. Zooplankton samples were collected via pump on days -3 and 11 and 38 days after the simulated dilbit spill. The zooplankton samples were co-extracted for DNA and RNA and were PCR amplified targeting the mitochondrial Cytochrome c Oxidase subunit I gene (CO1) region, with amplicon sequencing following. The dataset includes the demultiplexed sequencing output, the feature table with species-level taxonomic annotation, and the sample metadata. This dataset contains data from enclosures in rock and cobble substrate. Note that a similar study was conducted for wetland habitat enclosures, with different analyses and interpretation being conducted on the data (dataset available at https://doi.org/10.20383/101.0313).

Federated Research Data Repository / dépôt fédéré de données de recherche

Creative Commons Attribution 4.0 International (CC BY 4.0)

https://creativecommons.org/licenses/by/4.0/

metabarcoding

zooplankton

ecogenomics

Cytochrome c Oxidase subunit 1

diluted bitumen

remediation

boreal

freshwater

community RNA

RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures

f163c1b3-9c88-42f6-a7bb-5839ed6c4063

/6/published/publication_327/

AIP generation and transfer successful

2022-02-02

2021

2019-06-19/2019-07-30

Dataset

https://doi.org/10.1016/j.envpol.2021.118446

49.6964 -93.76752

ELA Lake 260;-;Ontario;Canada

Canada First Research Excellence Fund (CFREF)

419205

Global Water Futures Program (GWF)

Global Water Futures (GWF)

Global Water Futures (GWF)

Next generation solutions to ensure healthy water resources for future generations

Item exited embargo and became publicly available on 2021-12-01

L’élément est sorti de sa période d’embargo et est devenu public le 2021-12-01

Le jeu de données est sorti de sa période d’embargo et est devenu public le 2021-11-01