Field
Value
Language
dc.contributor.author
Ankley, Phillip
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0002-5883-6148
en_US
dc.contributor.author
Xie, Yuwei
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-5652-6413
en_US
dc.contributor.author
Havens, Sonya
datacite.creator.affiliationIdentifier
https://ror.org/043zcks33
en_US
datacite.creator.affiliation
International Institute for Sustainable Development
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Peters, Lisa
datacite.creator.affiliationIdentifier
https://ror.org/02gfys938
en_US
datacite.creator.affiliation
University of Manitoba
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0002-4310-3136
en_US
dc.contributor.author
Timlick, Lauren
datacite.creator.affiliationIdentifier
https://ror.org/043zcks33
en_US
datacite.creator.affiliation
International Institute for Sustainable Development
en_US
datacite.creator.nameIdentifier
en_US
dc.contributor.author
Rodriguez-Gil, Jose Luis
datacite.creator.affiliationIdentifier
https://ror.org/043zcks33
en_US
datacite.creator.affiliation
International Institute for Sustainable Development
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0001-9325-9131
en_US
dc.contributor.author
Giesy, John
datacite.creator.affiliationIdentifier
https://ror.org/010x8gc63
en_US
datacite.creator.affiliation
University of Saskatchewan
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-1931-9962
en_US
dc.contributor.author
Palace, Vince
datacite.creator.affiliationIdentifier
https://ror.org/043zcks33
en_US
datacite.creator.affiliation
International Institute for Sustainable Development
en_US
datacite.creator.nameIdentifier
https://orcid.org/0000-0003-2134-2299
en_US
dc.date.accessioned
2021-03-18T19:37:07Z
dc.date.available
2021-11-01T06:00:00Z
dc.date.issued
2021-11-01
dc.identifier.uri
https://www.frdr-dfdr.ca/repo/dataset/ea927258-85a7-451a-8ed8-fff82a690b7b
dc.identifier.uri
https://doi.org/10.20383/102.0332
dc.description
Zooplankton metabarcoding can provide a high-throughput method for characterizing community response to environmental stressors. There are issues, however, with inferring actual response to stressors using DNA metabarcoding as it cannot distinguish alive organisms within bulk samples. Here we used normalized vitality, namely RNA metabarcoding normalized by DNA metabarcoding, to characterize the zooplankton community response to environmental influence. DNA and RNA metabarcoding was also applied in the context of assessing response of the zooplankton community exposed to simulated spills of diluted bitumen (dilbit), with experimental remediation practices of enhanced monitored natural recovery and shoreline cleaner application. Zooplankton samples were collected via pump on days -3 and 11 and 38 days after the simulated dilbit spill. The zooplankton samples were co-extracted for DNA and RNA and were PCR amplified targeting the mitochondrial Cytochrome c Oxidase subunit I gene (CO1) region, with amplicon sequencing following. The dataset includes the demultiplexed sequencing output, the feature table with species-level taxonomic annotation, and the sample metadata. This dataset contains data from enclosures in rock and cobble substrate. Note that a similar study was conducted for wetland habitat enclosures, with different analyses and interpretation being conducted on the data (dataset available at https://doi.org/10.20383/101.0313).
en_US
dc.publisher
Federated Research Data Repository / dépôt fédéré de données de recherche
dc.rights
Creative Commons Attribution 4.0 International (CC BY 4.0)
en_US
dc.rights.uri
https://creativecommons.org/licenses/by/4.0/
en_US
dc.subject
metabarcoding
en_US
dc.subject
zooplankton
en_US
dc.subject
ecogenomics
en_US
dc.subject
Cytochrome c Oxidase subunit 1
en_US
dc.subject
diluted bitumen
en_US
dc.subject
remediation
en_US
dc.subject
boreal
en_US
dc.subject
freshwater
en_US
dc.subject
community RNA
en_US
dc.title
RNA metabarcoding to assess zooplankton community response to environmental influence in boreal lake experimental enclosures
en_US
globus.shared_endpoint.name
515c70c4-2eb8-4f2a-b406-7959b5edc28d
globus.shared_endpoint.path
/6/published/publication_327/
datacite.publicationYear
2021
datacite.date.Collected
2019-06-19/2019-07-30
datacite.resourceType
Dataset
en_US
datacite.relatedIdentifier.IsCitedBy
https://doi.org/10.1016/j.envpol.2021.118446
datacite.geolocation.geolocationPoint
49.6964 -93.76752
datacite.geolocation.geolocationPlace
ELA Lake 260;-;Ontario;Canada
datacite.fundingReference.funderName
Canada First Research Excellence Fund (CFREF)
en_US
datacite.fundingReference.awardNumber
419205
en_US
datacite.fundingReference.awardTitle
Global Water Futures Program (GWF)
en_US
datacite.fundingReference.funderName
Global Water Futures (GWF)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
en_US
datacite.fundingReference.funderName
Global Water Futures (GWF)
en_US
datacite.fundingReference.awardNumber
en_US
datacite.fundingReference.awardTitle
Next generation solutions to ensure healthy water resources for future generations
en_US
frdr.crdc.code
RDF1060312
frdr.crdc.group_en
Biological sciences
en_US
frdr.crdc.class_en
Ecology (except applications)
en_US
frdr.crdc.field_en
Environmental metagenomics
en_US
frdr.crdc.group_fr
Sciences biologiques
fr_CA
frdr.crdc.class_fr
Écologie (sauf les applications)
fr_CA
frdr.crdc.field_fr
Métagénomique environnementale
fr_CA
datacite.description.other
Item exited embargo and became publicly available on 2021-12-01
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