Description:	L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need other substrates. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate and 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90% serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.
Authors:	Huang, Po-Jung Jimmy; University of Waterloo; 0000-0003-3436-9968 Liu, Juewen; University of Waterloo; 0000-0001-5918-9336
Keywords:	DNA SELEX Aptamer L-Lactate D-Glucose Biosensor
Field of Research:	Biological sciences > Biochemistry > Analytical biochemistry
Publication Date:	2023-03-09
Publisher:	Federated Research Data Repository / dépôt fédéré de données de recherche
Funder:	Natural Sciences and Engineering Research Council Canada First Research Excellence Fund (CFREF)
URI:	https://doi.org/10.20383/102.0690
Related Identifiers:	This dataset is cited by https://doi.org/10.1002/anie.202212879
Geographic Coverage:	Place Name University of Waterloo City Waterloo Province / Province / Territory Ontario Territory   Country Canada
Appears in Collections:	Water Institute