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Simultaneous Detection of L-Lactate and D-Glucose Using DNA Aptamers in Human Blood Serum
| Description: | L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need other substrates. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate and 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90% serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring. |
| Authors: | Huang, Po-Jung Jimmy; University of Waterloo;  0000-0003-3436-9968Liu, Juewen; University of Waterloo; 0000-0001-5918-9336 |
| Keywords: | DNA SELEX Aptamer L-Lactate D-Glucose Biosensor |
| Field of Research: | Biological sciences > Biochemistry > Analytical biochemistry
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| Publication Date: | 2023-03-09 |
| Publisher: | Federated Research Data Repository / dépôt fédéré de données de recherche |
| Funder: | Natural Sciences and Engineering Research Council of Canada Canada First Research Excellence Fund (CFREF) |
| URI: | https://doi.org/10.20383/102.0690 |
| Related Identifiers: |
This dataset is cited by
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| Geographic Coverage: | Place Name University of Waterloo City Waterloo Province / Province / Territory Ontario Territory Country Canada |
| Appears in Collections: | Water Institute |
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Creative Commons Public Domain Dedication (CC0 1.0) https://creativecommons.org/publicdomain/zero/1.0/
Citation
Huang, P., Liu, J. (2023). Simultaneous Detection of L-Lactate and D-Glucose Using DNA Aptamers in Human Blood Serum. Federated Research Data Repository. https://doi.org/10.20383/102.0690
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