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Simultaneous Detection of L-Lactate and D-Glucose Using DNA Aptamers in Human Blood Serum

Description: L-lactate is a key metabolite indicative of physiological states, glycolysis pathways, and various diseases such as sepsis, heart attack, lactate acidosis, and cancer. Detection of lactate has been relying on a few enzymes that need other substrates. In this work, DNA aptamers for L-lactate were obtained using a library-immobilization selection method and the highest affinity aptamer reached a Kd of 0.43 mM as determined using isothermal titration calorimetry. The aptamers showed up to 50-fold selectivity for L-lactate over D-lactate and had little responses to other closely related analogs such as pyruvate and 3-hydroxybutyrate. A fluorescent biosensor based on the strand displacement method showed a limit of detection of 0.55 mM L-lactate, and the sensor worked in 90% serum. Simultaneous detection of L-lactate and D-glucose in the same solution was achieved. This work has broadened the scope of aptamers to simple metabolites and provided a useful probe for continuous and multiplexed monitoring.
Authors: Huang, Po-Jung Jimmy; University of Waterloo; ORCID iD 0000-0003-3436-9968
Liu, Juewen; University of Waterloo; ORCID iD 0000-0001-5918-9336
Keywords: DNA
Field of Research: 
Biological sciences
Analytical biochemistry
Publication Date: 2023-03-09
Publisher: Federated Research Data Repository / dépôt fédéré de données de recherche
Funder: Natural Sciences and Engineering Research Council of Canada
Canada First Research Excellence Fund (CFREF)
Related Identifiers: 
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Geographic Coverage: 
Place Name
University of Waterloo
Province /
Province / Territory
Appears in Collections:Water Institute

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Access to this dataset is subject to the following terms:
Creative Commons Public Domain Dedication (CC0 1.0)
Huang, P., Liu, J. (2023). Simultaneous Detection of L-Lactate and D-Glucose Using DNA Aptamers in Human Blood Serum. Federated Research Data Repository.